Detailed information of TA system TA062666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4688180..4688782
• Replicon: chromosome
• Accession: NZ_CP015228
• Organism: Escherichia coli strain 09-00049

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GJ12_RS25150
• Protein ID: WP_000897305.1
• Coordinates: 4688180..4688491 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GJ12_RS25155
• Protein ID: WP_000356397.1
• Coordinates: 4688492..4688782 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ12_RS25120	4683210..4683995	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
GJ12_RS25125	4684094..4684693	+	600	WP_001315111.1	glucose-1-phosphatase	-
GJ12_RS25130	4684687..4685559	+	873	WP_000920762.1	virulence factor BrkB family protein	-
GJ12_RS25135	4685556..4685993	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GJ12_RS25140	4686038..4686979	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GJ12_RS25145	4687043..4687951	-	909	WP_001162704.1	alpha/beta hydrolase	-
GJ12_RS25150	4688180..4688491	+	312	WP_000897305.1	hypothetical protein	Toxin
GJ12_RS25155	4688492..4688782	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GJ12_RS25160	4689387..4689605	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GJ12_RS25165	4689824..4690066	+	243	WP_001086388.1	hypothetical protein	-
GJ12_RS25175	4690396..4691325	-	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
GJ12_RS25180	4691322..4691957	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GJ12_RS25185	4691954..4692856	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T62666 WP_000897305.1 NZ_CP015228:4688180-4688491 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T62666 NZ_CP015228:4688180-4688491 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT62666 WP_000356397.1 NZ_CP015228:4688492-4688782 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT62666 NZ_CP015228:4688492-4688782 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7