Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 289171..289712 Replicon chromosome
Accession NZ_CP015228
Organism Escherichia coli strain 09-00049

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag GJ12_RS02555 Protein ID WP_000615982.1
Coordinates 289171..289449 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GJ12_RS02560 Protein ID WP_000729704.1
Coordinates 289452..289712 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ12_RS02540 286753..287331 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
GJ12_RS02545 287537..288304 + 768 WP_065225510.1 class II glutamine amidotransferase -
GJ12_RS02550 288275..289015 - 741 WP_001225679.1 murein L,D-transpeptidase -
GJ12_RS02555 289171..289449 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GJ12_RS02560 289452..289712 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GJ12_RS02565 289898..290671 + 774 WP_122992790.1 C40 family peptidase -
GJ12_RS02570 290728..291084 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
GJ12_RS02575 291077..291355 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GJ12_RS02580 291460..293172 - 1713 Protein_254 flagellar biosynthesis protein FlhA -
GJ12_RS02585 293144..293929 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T62648 WP_000615982.1 NZ_CP015228:c289449-289171 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T62648 NZ_CP015228:c289449-289171 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT62648 WP_000729704.1 NZ_CP015228:c289712-289452 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT62648 NZ_CP015228:c289712-289452 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References