Detailed information of TA system TA062634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1551082..1551812
• Replicon: chromosome
• Accession: NZ_CP015227
• Organism: Enterobacter sp. ODB01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: A4308_RS07400
• Protein ID: WP_045134487.1
• Coordinates: 1551082..1551396 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A4308_RS07405
• Protein ID: WP_023331687.1
• Coordinates: 1551393..1551812 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4308_RS07375	1546747..1547835	+	1089	WP_032635252.1	cellulase family glycosylhydrolase	-
A4308_RS07380	1547738..1548922	-	1185	WP_032637792.1	multidrug efflux MFS transporter EmrD	-
A4308_RS07385	1549098..1549931	-	834	WP_045281350.1	DMT family transporter	-
A4308_RS07390	1549994..1550440	-	447	WP_063308488.1	GNAT family N-acetyltransferase	-
A4308_RS21700	1550505..1550594	-	90	WP_045281349.1	type I toxin-antitoxin system toxin TisB	-
A4308_RS07400	1551082..1551396	+	315	WP_045134487.1	type II toxin-antitoxin system HigB family toxin	Toxin
A4308_RS07405	1551393..1551812	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
A4308_RS22395	1551962..1552060	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
A4308_RS07410	1552167..1553855	+	1689	WP_023331689.1	acetolactate synthase large subunit	-
A4308_RS07415	1553859..1554146	+	288	WP_010426528.1	acetolactate synthase small subunit	-
A4308_RS07420	1554229..1554822	+	594	WP_014881931.1	transcriptional regulator UhpA	-
A4308_RS07425	1554819..1556324	+	1506	WP_045281347.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12455.66 Da
• Isoelectric Point: 10.3843

>T62634 WP_045134487.1 NZ_CP015227:1551082-1551396 [Enterobacter sp. ODB01]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNKLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T62634 NZ_CP015227:1551082-1551396 [Enterobacter sp. ODB01]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCACGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAAACTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT62634 WP_023331687.1 NZ_CP015227:1551393-1551812 [Enterobacter sp. ODB01]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT62634 NZ_CP015227:1551393-1551812 [Enterobacter sp. ODB01]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA