Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4406438..4407024 Replicon chromosome
Accession NZ_CP015202
Organism Pseudomonas putida B6-2

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag KKK_RS30045 Protein ID WP_029380715.1
Coordinates 4406438..4406716 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag KKK_RS19925 Protein ID WP_003252274.1
Coordinates 4406725..4407024 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKK_RS19915 4403239..4404435 + 1197 WP_019752412.1 succinyldiaminopimelate transaminase -
KKK_RS19920 4404541..4406187 - 1647 WP_012053546.1 Na+/H+ antiporter -
KKK_RS30045 4406438..4406716 + 279 WP_029380715.1 type II toxin-antitoxin system toxin GraT Toxin
KKK_RS19925 4406725..4407024 + 300 WP_003252274.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
KKK_RS19930 4407149..4407496 + 348 WP_003252272.1 ArsC family reductase -
KKK_RS19935 4407528..4408562 + 1035 WP_019752413.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
KKK_RS19940 4408644..4409849 + 1206 WP_019752414.1 cysteine desulfurase -
KKK_RS19945 4409846..4410256 + 411 WP_003252266.1 SufE family protein -
KKK_RS19950 4410365..4411174 - 810 WP_003252264.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-90)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10501.84 Da        Isoelectric Point: 7.2448

>T62615 WP_029380715.1 NZ_CP015202:4406438-4406716 [Pseudomonas putida B6-2]
MIQSFSCADTEALFTTGKTRRWSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRAGQHSIRVNDQWRLCFIWTE
HGPVNVEIVDYH

Download         Length: 279 bp

>T62615 NZ_CP015202:4406438-4406716 [Pseudomonas putida B6-2]
ATGATTCAAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGCTGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAACTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCTCCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGGCCAACACAGTATCCGTGTAAATGACCAATGGCGGTTGTGCTTCATCTGGACAGAG
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10891.52 Da        Isoelectric Point: 5.6714

>AT62615 WP_003252274.1 NZ_CP015202:4406725-4407024 [Pseudomonas putida B6-2]
MLKNGMRPIHPGEILREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp

>AT62615 NZ_CP015202:4406725-4407024 [Pseudomonas putida B6-2]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGACTTCCAAAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGTCTGTCCATCTGCCTGGACACCACCCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGCCTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References