Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type III Classification (family/domain) toxIN/ToxN(toxin)
Location 62718..63460 Replicon plasmid pBMB71
Accession NZ_CP015180
Organism Bacillus thuringiensis serovar alesti strain BGSC 4C1

Toxin (Protein)


Gene name toxN Uniprot ID Q3YN09
Locus tag Bt4C1_RS29335 Protein ID WP_000182254.1
Coordinates 62718..63302 (-) Length 195 a.a.

Antitoxin (RNA)


Gene name toxI
Locus tag -
Coordinates 63364..63460 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Bt4C1_RS29315 58123..58569 - 447 WP_001101375.1 CPBP family intramembrane metalloprotease -
Bt4C1_RS29320 58599..60740 - 2142 WP_000209240.1 type IA DNA topoisomerase -
Bt4C1_RS29325 60862..61242 - 381 WP_000517026.1 hypothetical protein -
Bt4C1_RS29330 61349..62650 - 1302 WP_000877084.1 hypothetical protein -
Bt4C1_RS29335 62718..63302 - 585 WP_000182254.1 type III toxin-antitoxin system ToxN/AbiQ family toxin Toxin
- 63364..63460 - 97 NuclAT_0 - Antitoxin
- 63364..63460 - 97 NuclAT_0 - Antitoxin
- 63364..63460 - 97 NuclAT_0 - Antitoxin
- 63364..63460 - 97 NuclAT_0 - Antitoxin
Bt4C1_RS29340 63553..64578 - 1026 WP_000437709.1 DUF3991 and TOPRIM domain-containing protein -
Bt4C1_RS29345 64658..65209 - 552 WP_000875863.1 metal-dependent hydrolase -
Bt4C1_RS29350 65233..66159 - 927 WP_000131507.1 hypothetical protein -
Bt4C1_RS29355 66222..67394 - 1173 WP_002134161.1 hypothetical protein -
Bt4C1_RS29360 67391..67600 - 210 WP_000082222.1 DUF5511 family protein -
Bt4C1_RS29365 67623..68051 - 429 WP_000066903.1 DUF5514 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..71696 71696


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ToxN/AbiQ toxin

(8-169)


Sequences

Toxin        


Download         Length: 195 a.a.        Molecular weight: 22887.13 Da        Isoelectric Point: 8.9873

>T62596 WP_000182254.1 NZ_CP015180:c63302-62718 [Bacillus thuringiensis serovar alesti]
MTNKDNPKFHTISTEYIDYLREADSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDNDEKRIG
VLLVNNMIPVPEKECKEIDIAEKTAADPQYGNLMLKQYLFLKENMDRVTNKVEKVYKDVTVQGKPSHKQKFLKGVCCDFP
KLEEKCQEYKERDQAKERDKARRIAYMRQMGRER

Download         Length: 585 bp

>T62596 NZ_CP015180:c63302-62718 [Bacillus thuringiensis serovar alesti]
TTGACTAATAAAGATAATCCTAAATTTCACACTATTTCAACTGAATATATAGATTATTTAAGAGAAGCGGATAGTAAAGT
ACCTTTTAATAAAGATGAACAGCATAGCAGACCGTATGTAGGTGTATTGGAAAAAATCAATGGTCATGATTATTTTGTAC
CTTTAACATCACGTAACGATAAAAATTTTAACAGTCAGGTCAGTGTAAAGTTATTCGATAATGATGAAAAGCGAATTGGA
GTTTTATTAGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAACAGCAGCAGA
CCCTCAGTATGGTAATTTGATGTTAAAACAGTATTTATTTTTAAAAGAAAATATGGATCGAGTTACTAATAAAGTGGAAA
AAGTATATAAAGATGTAACTGTACAAGGAAAGCCTTCACATAAACAAAAATTCTTAAAAGGAGTTTGTTGTGATTTCCCT
AAATTAGAAGAAAAGTGCCAAGAATATAAAGAAAGAGATCAAGCTAAAGAAAGAGATAAAGCTAGAAGAATAGCATATAT
GCGTCAAATGGGGCGTGAGAGATAA

Antitoxin


Download         Length: 97 bp

>AT62596 NZ_CP015180:c63460-63364 [Bacillus thuringiensis serovar alesti]
GTAACCTTACTTTAGTAGGTGCTAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCTAAAAAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ATO

Antitoxin

Download structure file

References