Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 14974..15515 Replicon plasmid unnamed3
Accession NZ_CP015171
Organism Acetobacter ascendens strain LMG 1591

Toxin (Protein)


Gene name yoeB Uniprot ID G2I8S5
Locus tag A4R89_RS15725 Protein ID WP_014106724.1
Coordinates 14974..15243 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID G2I8S6
Locus tag A4R89_RS15730 Protein ID WP_010668421.1
Coordinates 15240..15515 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R89_RS15710 10338..11723 - 1386 WP_070325023.1 IS1380-like element IS1380A family transposase -
A4R89_RS15715 11819..14164 + 2346 Protein_8 restriction endonuclease subunit R -
A4R89_RS15720 14290..14961 - 672 WP_173572193.1 hypothetical protein -
A4R89_RS15725 14974..15243 - 270 WP_014106724.1 Txe/YoeB family addiction module toxin Toxin
A4R89_RS15730 15240..15515 - 276 WP_010668421.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
A4R89_RS15735 15741..15878 - 138 WP_070325623.1 plasmid stabilization protein -
A4R89_RS15740 15904..16296 - 393 WP_010668423.1 PIN domain nuclease -
A4R89_RS15745 16293..16487 - 195 WP_010516209.1 type II toxin-antitoxin system VapB family antitoxin -
A4R89_RS15750 16542..19628 - 3087 WP_070325624.1 Ti-type conjugative transfer relaxase TraA -
A4R89_RS15755 19725..20084 + 360 WP_211274218.1 conjugal transfer protein TraD -
A4R89_RS15760 20149..20388 + 240 WP_070325633.1 conjugal transfer protein TraD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..28186 28186
- flank IS/Tn - - 10338..11723 1385


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-89)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10563.91 Da        Isoelectric Point: 7.3582

>T62578 WP_014106724.1 NZ_CP015171:c15243-14974 [Acetobacter ascendens]
VKVIFHEDGWEDYLSWFQSDNAVLEKINALIEDVRRHPFQGIGKPEPLKGQLSGWWSRRITGEHRLVYRVQGRAGEDQRI
EIAQCRFHY

Download         Length: 270 bp

>T62578 NZ_CP015171:c15243-14974 [Acetobacter ascendens]
GTGAAAGTCATTTTTCATGAAGATGGCTGGGAGGATTATCTTTCGTGGTTTCAGTCAGACAATGCGGTTCTTGAGAAGAT
CAATGCCCTGATCGAGGATGTGCGTCGGCATCCGTTTCAGGGGATAGGGAAGCCGGAACCCCTGAAGGGGCAGCTTTCTG
GGTGGTGGTCCCGACGGATTACGGGAGAACATCGTCTGGTCTACCGGGTTCAGGGGCGCGCGGGAGAGGATCAGCGGATC
GAGATTGCCCAGTGCCGGTTTCATTACTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10206.37 Da        Isoelectric Point: 5.1070

>AT62578 WP_010668421.1 NZ_CP015171:c15515-15240 [Acetobacter ascendens]
MTYVSYTDLRQNLAHYLDEAVNSRAPIVVTRKTGKGSVVLMSEEEFSGWQETVHLLSSPRNAERLLRSIRDIEHGAATEH
DLLEPQGEPSA

Download         Length: 276 bp

>AT62578 NZ_CP015171:c15515-15240 [Acetobacter ascendens]
ATGACCTATGTTTCCTATACCGACCTGCGCCAGAACCTGGCGCATTATCTGGACGAAGCAGTGAACAGCCGTGCGCCGAT
TGTCGTGACCCGCAAGACCGGGAAAGGCAGTGTCGTGCTGATGTCGGAAGAAGAATTCTCCGGCTGGCAGGAAACCGTGC
ATCTCCTGAGCAGCCCACGCAACGCGGAGAGGCTGTTGCGGAGCATTCGTGACATCGAGCACGGGGCGGCAACCGAGCAT
GATCTTCTGGAGCCTCAGGGTGAACCGTCAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A149R123

Antitoxin

Source ID Structure
AlphaFold DB A0A149R124

References