Detailed information of TA system TA062558

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 183749..184171
• Replicon: plasmid pECO-fce
• Accession: NZ_CP015160
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: WM90_RS26020
• Protein ID: WP_001302184.1
• Coordinates: 183749..183907 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	183972..184171 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS25980	178793..179020	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
WM90_RS25985	179114..179800	-	687	WP_000332487.1	PAS domain-containing protein	-
WM90_RS25990	179994..180377	-	384	WP_001151562.1	relaxosome protein TraM	-
WM90_RS25995	180708..181310	+	603	WP_001323326.1	transglycosylase SLT domain-containing protein	-
WM90_RS26000	181606..182427	-	822	WP_001234489.1	DUF945 domain-containing protein	-
WM90_RS26005	182546..182833	-	288	WP_000107526.1	hypothetical protein	-
WM90_RS26020	183749..183907	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	183972..184171	-	200	NuclAT_1	-	Antitoxin
-	183972..184171	-	200	NuclAT_1	-	Antitoxin
-	183972..184171	-	200	NuclAT_1	-	Antitoxin
-	183972..184171	-	200	NuclAT_1	-	Antitoxin
WM90_RS26025	184183..184902	-	720	WP_001276230.1	plasmid SOS inhibition protein A	-
WM90_RS26030	184899..185333	-	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
WM90_RS26035	185388..187346	-	1959	WP_001145501.1	ParB/RepB/Spo0J family partition protein	-
WM90_RS26040	187405..187638	-	234	WP_000006018.1	DUF905 domain-containing protein	-
WM90_RS26045	187695..188222	-	528	WP_000290833.1	single-stranded DNA-binding protein	-
WM90_RS29620	188524..188976	+	453	WP_024185774.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B	senB	1..212180	212180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T62558 WP_001302184.1 NZ_CP015160:c183907-183749 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T62558 NZ_CP015160:c183907-183749 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 200 bp

>AT62558 NZ_CP015160:c184171-183972 [Escherichia coli]
TCACAACGGATTGCCCGTAAACAGCCTGAATGAGCGAGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATAT
GGGCTATGATGTGCCCGGCGCTTGAGGTTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGG
ACAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin