Detailed information of TA system TA062554

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 143172..143425
• Replicon: plasmid pECO-fce
• Accession: NZ_CP015160
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: WM90_RS25795
• Protein ID: WP_001336447.1
• Coordinates: 143172..143321 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	143369..143425 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS25760	139147..139812	+	666	WP_001535733.1	hypothetical protein	-
WM90_RS25765	139848..140147	-	300	WP_001528606.1	type II toxin-antitoxin system RelE/ParE family toxin	-
WM90_RS25770	140137..140400	-	264	WP_001089473.1	hypothetical protein	-
WM90_RS29600	140507..140767	-	261	WP_019842130.1	hypothetical protein	-
WM90_RS25780	141470..142327	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
WM90_RS30250	142320..142394	-	75	WP_001365705.1	RepA leader peptide Tap	-
WM90_RS25790	142630..142887	-	258	WP_000084404.1	replication regulatory protein RepA	-
WM90_RS25795	143172..143321	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	143369..143425	+	57	NuclAT_2	-	Antitoxin
-	143369..143425	+	57	NuclAT_2	-	Antitoxin
-	143369..143425	+	57	NuclAT_2	-	Antitoxin
-	143369..143425	+	57	NuclAT_2	-	Antitoxin
WM90_RS25800	143520..143957	-	438	WP_000872609.1	hypothetical protein	-
WM90_RS30715	144110..144494	-	385	Protein_180	hypothetical protein	-
WM90_RS25810	144597..145058	-	462	WP_019842128.1	thermonuclease family protein	-
WM90_RS28730	145800..146003	-	204	WP_001336517.1	hypothetical protein	-
WM90_RS25815	146155..146712	-	558	WP_000139329.1	fertility inhibition protein FinO	-
WM90_RS25820	146767..147513	-	747	WP_000205725.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B	senB	1..212180	212180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T62554 WP_001336447.1 NZ_CP015160:c143321-143172 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T62554 NZ_CP015160:c143321-143172 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT62554 NZ_CP015160:143369-143425 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin