Detailed information of TA system TA062550

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 116699..117125
• Replicon: plasmid pECO-fce
• Accession: NZ_CP015160
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: WM90_RS25595
• Protein ID: WP_001312861.1
• Coordinates: 116699..116857 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	116901..117125 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS25560	112073..112762	-	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-
WM90_RS25565	112949..113332	-	384	WP_001151538.1	relaxosome protein TraM	-
WM90_RS25570	113653..114255	+	603	Protein_137	transglycosylase SLT domain-containing protein	-
WM90_RS25575	114551..115354	-	804	WP_162780142.1	DUF945 domain-containing protein	-
WM90_RS25580	115478..115765	-	288	WP_000107526.1	hypothetical protein	-
WM90_RS30705	116066..116461	+	396	Protein_140	hypothetical protein	-
WM90_RS25595	116699..116857	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	116901..117125	-	225	NuclAT_0	-	Antitoxin
-	116901..117125	-	225	NuclAT_0	-	Antitoxin
-	116901..117125	-	225	NuclAT_0	-	Antitoxin
-	116901..117125	-	225	NuclAT_0	-	Antitoxin
WM90_RS30510	116937..117116	+	180	WP_001309233.1	hypothetical protein	-
WM90_RS25605	117137..117856	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
WM90_RS25610	117853..118287	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
WM90_RS25615	118356..120320	-	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
WM90_RS25620	120381..120614	-	234	WP_000006003.1	DUF905 family protein	-
WM90_RS25625	120672..121199	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B	senB	1..212180	212180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T62550 WP_001312861.1 NZ_CP015160:c116857-116699 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T62550 NZ_CP015160:c116857-116699 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT62550 NZ_CP015160:c117125-116901 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin