Detailed information of TA system TA062499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 122422..123079
• Replicon: plasmid pEC732_IMP14
• Accession: NZ_CP015139
• Organism: Escherichia coli strain Ecol_732

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: A4X18_RS25690
• Protein ID: WP_000270043.1
• Coordinates: 122422..122772 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A4X18_RS25695
• Protein ID: WP_000124640.1
• Coordinates: 122777..123079 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4X18_RS25650	118896..119393	-	498	WP_000062185.1	hypothetical protein	-
A4X18_RS25655	119396..119884	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
A4X18_RS25660	119981..120316	+	336	WP_000683477.1	hypothetical protein	-
A4X18_RS25665	120331..120801	-	471	WP_001281821.1	hypothetical protein	-
A4X18_RS25670	120794..121165	-	372	WP_000516918.1	hypothetical protein	-
A4X18_RS25675	121176..121370	-	195	WP_000343597.1	hypothetical protein	-
A4X18_RS25685	121711..122259	-	549	WP_001061573.1	hypothetical protein	-
A4X18_RS25690	122422..122772	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4X18_RS25695	122777..123079	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
A4X18_RS25700	123106..123399	-	294	WP_001239998.1	hypothetical protein	-
A4X18_RS25705	123488..123760	-	273	WP_001043046.1	HU family DNA-binding protein	-
A4X18_RS25710	123818..124345	-	528	WP_001210659.1	thermonuclease family protein	-
A4X18_RS31540	124348..124653	-	306	WP_171820680.1	hypothetical protein	-
A4X18_RS25720	124565..125422	-	858	WP_001167036.1	hypothetical protein	-
A4X18_RS25725	125409..125639	-	231	WP_000972665.1	hypothetical protein	-
A4X18_RS25730	125639..126157	-	519	WP_000210757.1	nitrite reductase	-
A4X18_RS25735	126154..126600	-	447	WP_000919343.1	hypothetical protein	-
A4X18_RS25740	126600..126959	-	360	WP_000422769.1	hypothetical protein	-
A4X18_RS25745	127017..127445	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / ant(2'')-Ia / blaIMP-14 / aph(3')-VIa	-	1..186826	186826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T62499 WP_000270043.1 NZ_CP015139:122422-122772 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T62499 NZ_CP015139:122422-122772 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT62499 WP_000124640.1 NZ_CP015139:122777-123079 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT62499 NZ_CP015139:122777-123079 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9