Detailed information of TA system TA062484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 3801431..3801933
• Replicon: chromosome
• Accession: NZ_CP015138
• Organism: Escherichia coli strain Ecol_732

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: A4X18_RS18525
• Protein ID: WP_000767819.1
• Coordinates: 3801679..3801933 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: A4X18_RS18520
• Protein ID: WP_001259253.1
• Coordinates: 3801431..3801682 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4X18_RS18500	3796609..3797676	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
A4X18_RS18505	3797676..3798746	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
A4X18_RS18510	3798743..3800047	-	1305	WP_001296211.1	histidinol dehydrogenase	-
A4X18_RS18515	3800053..3800952	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
A4X18_RS28700	3801098..3801148	-	51	WP_001364200.1	his operon leader peptide	-
A4X18_RS18520	3801431..3801682	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
A4X18_RS18525	3801679..3801933	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
A4X18_RS18530	3802016..3802840	+	825	WP_000754737.1	SDR family oxidoreductase	-
A4X18_RS18535	3802886..3803815	+	930	WP_000803366.1	LysR family transcriptional regulator	-
A4X18_RS30725	3804030..3804092	+	63	WP_010723108.1	membrane protein YoeI	-
A4X18_RS18540	3804082..3805440	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
A4X18_RS18550	3805657..3806115	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3805657..3806115	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T62484 WP_000767819.1 NZ_CP015138:3801679-3801933 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T62484 NZ_CP015138:3801679-3801933 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT62484 WP_001259253.1 NZ_CP015138:3801431-3801682 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT62484 NZ_CP015138:3801431-3801682 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3