Detailed information of TA system TA062467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4810040..4810734
• Replicon: chromosome
• Accession: NZ_CP015137
• Organism: Dickeya solani IPO 2222

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: A4U42_RS20565
• Protein ID: WP_026594479.1
• Coordinates: 4810040..4810438 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: A0A2K8VUD1
• Locus tag: A4U42_RS20570
• Protein ID: WP_022633740.1
• Coordinates: 4810441..4810734 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4U42_RS20535	4805220..4806254	-	1035	WP_022633732.1	harpin HrpZ family protein	-
A4U42_RS20540	4806415..4806825	-	411	WP_022633733.1	hypothetical protein	-
A4U42_RS20545	4806822..4807010	-	189	WP_022633734.1	hypothetical protein	-
A4U42_RS20550	4807045..4809114	-	2070	WP_023637836.1	type III secretion system outer membrane ring subunit SctC	-
A4U42_RS20555	4809107..4809541	-	435	WP_022633737.1	hypothetical protein	-
A4U42_RS20560	4809528..4809755	-	228	WP_022633738.1	type III secretion protein HrpF	-
A4U42_RS20565	4810040..4810438	-	399	WP_026594479.1	type II toxin-antitoxin system YafO family toxin	Toxin
A4U42_RS20570	4810441..4810734	-	294	WP_022633740.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A4U42_RS20575	4810952..4812001	-	1050	WP_022633741.1	hypothetical protein	-
A4U42_RS20580	4812541..4813701	+	1161	WP_022633742.1	alpha/beta fold hydrolase	-
A4U42_RS20585	4813712..4815025	-	1314	WP_022633743.1	lytic murein transglycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4809528..4819456	9928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15230.56 Da
• Isoelectric Point: 8.4931

>T62467 WP_026594479.1 NZ_CP015137:c4810438-4810040 [Dickeya solani IPO 2222]
IRIFKSTLIRQQLSQQELDDLVADFHAYKQNGVLPDTFGRDAPYDDDRTYPLVKAEQVAHIHLADADAPFPRFLRQFKRT
SDRAHLVYCQGAMDPEACLLIIILKPEAHKMARNNNNMHKIGMMAAAFRMKY

Nucleotide

• Length: 399 bp

>T62467 NZ_CP015137:c4810438-4810040 [Dickeya solani IPO 2222]
ATACGGATTTTCAAGTCAACGCTGATCCGTCAACAATTGAGCCAGCAAGAGCTTGATGATTTGGTCGCGGATTTTCACGC
CTATAAGCAGAATGGGGTACTACCGGACACATTTGGGCGTGACGCGCCTTATGATGATGACCGAACCTATCCATTAGTAA
AAGCAGAGCAGGTGGCTCACATCCACCTTGCCGATGCGGATGCGCCTTTTCCCAGGTTCTTGCGCCAGTTCAAGAGAACC
AGCGATCGGGCCCATTTGGTCTACTGCCAGGGAGCGATGGATCCCGAGGCCTGCCTGTTAATTATTATTTTAAAACCCGA
AGCCCATAAAATGGCACGCAACAATAACAACATGCACAAGATCGGGATGATGGCCGCTGCGTTCAGGATGAAATATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10934.48 Da
• Isoelectric Point: 5.2082

>AT62467 WP_022633740.1 NZ_CP015137:c4810734-4810441 [Dickeya solani IPO 2222]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEAKQETVHTVARFRPTAERLRALADSG
QKLLNDASGKDLTEFTE

Nucleotide

• Length: 294 bp

>AT62467 NZ_CP015137:c4810734-4810441 [Dickeya solani IPO 2222]
ATGGAGCGTATTCTTGCTGAAAAATCTATAAATATTACAGAGTTAAGGAAAAATCCGGCTAAGTATTTTATTGATGAGCC
TGTTGCCGTGCTTTCTAACAACCGGCCCGCCGGTTACATGGTGAGTGCCAAAGTCTTTGAAGAACTGATCGATCTGCTAG
AGGCGAAGCAGGAAACGGTTCATACCGTAGCGCGCTTTAGGCCAACGGCCGAACGTTTACGAGCCCTTGCAGATAGCGGT
CAGAAACTGCTGAATGATGCCTCTGGCAAGGATCTGACTGAGTTCACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8VUD1