Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2621956..2622553 Replicon chromosome
Accession NZ_CP015134
Organism Klebsiella pneumoniae strain ATCC 35657

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag WM93_RS13540 Protein ID WP_004142563.1
Coordinates 2621956..2622273 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag WM93_RS13545 Protein ID WP_004142561.1
Coordinates 2622266..2622553 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM93_RS13520 2617660..2619087 - 1428 WP_075394659.1 MFS transporter -
WM93_RS13525 2619196..2620062 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
WM93_RS13535 2620728..2621771 + 1044 WP_075394660.1 DUF2157 domain-containing protein -
WM93_RS13540 2621956..2622273 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
WM93_RS13545 2622266..2622553 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
WM93_RS13550 2622818..2623471 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
WM93_RS13555 2623591..2623959 - 369 WP_002893024.1 MmcQ/YjbR family DNA-binding protein -
WM93_RS13560 2623959..2624540 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
WM93_RS13565 2624720..2625835 + 1116 WP_075394661.1 MBL fold metallo-hydrolase -
WM93_RS13570 2625866..2626207 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
WM93_RS13575 2626225..2626473 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T62433 WP_004142563.1 NZ_CP015134:2621956-2622273 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T62433 NZ_CP015134:2621956-2622273 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT62433 WP_004142561.1 NZ_CP015134:2622266-2622553 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT62433 NZ_CP015134:2622266-2622553 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References