Detailed information of TA system TA062423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4649578..4650175
• Replicon: chromosome
• Accession: NZ_CP015130
• Organism: Klebsiella pneumoniae strain Kpn555

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: WM92_RS23170
• Protein ID: WP_064840127.1
• Coordinates: 4649578..4649895 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: WM92_RS23175
• Protein ID: WP_004142561.1
• Coordinates: 4649888..4650175 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM92_RS23155	4645281..4646708	-	1428	WP_064840124.1	MFS transporter	-
WM92_RS23160	4646817..4647683	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
WM92_RS23165	4648350..4649393	+	1044	WP_064840125.1	DUF2157 domain-containing protein	-
WM92_RS23170	4649578..4649895	+	318	WP_064840127.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WM92_RS23175	4649888..4650175	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
WM92_RS23180	4650440..4651093	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
WM92_RS23185	4651213..4651581	-	369	WP_040171587.1	MmcQ/YjbR family DNA-binding protein	-
WM92_RS23190	4651578..4652162	-	585	WP_002893026.1	TetR/AcrR family transcriptional regulator	-
WM92_RS23195	4652342..4653457	+	1116	WP_074193502.1	MBL fold metallo-hydrolase	-
WM92_RS23200	4653488..4653829	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
WM92_RS23205	4653847..4654095	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12182.45 Da
• Isoelectric Point: 11.1076

>T62423 WP_064840127.1 NZ_CP015130:4649578-4649895 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQELFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T62423 NZ_CP015130:4649578-4649895 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
ACAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGAGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT62423 WP_004142561.1 NZ_CP015130:4649888-4650175 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT62423 NZ_CP015130:4649888-4650175 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1