Detailed information of TA system TA062382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2554269..2554864
• Replicon: chromosome
• Accession: NZ_CP015117
• Organism: Pseudomonas aeruginosa strain ATCC 27853

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: A4W92_RS32270
• Protein ID: WP_003117425.1
• Coordinates: 2554586..2554864 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A4W92_RS11950
• Protein ID: WP_003099268.1
• Coordinates: 2554269..2554574 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4W92_RS11930	2549722..2550012	-	291	WP_023083237.1	DUF5447 family protein	-
A4W92_RS11940	2550224..2550496	-	273	WP_003115921.1	hypothetical protein	-
A4W92_RS11945	2550606..2550872	+	267	WP_016852153.1	hypothetical protein	-
A4W92_RS32255	2551046..2551840	+	795	WP_023082695.1	helix-turn-helix domain-containing protein	-
A4W92_RS32260	2551815..2553353	+	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
A4W92_RS32265	2553365..2553892	-	528	WP_071535723.1	AAA family ATPase	-
A4W92_RS11950	2554269..2554574	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
A4W92_RS32270	2554586..2554864	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4W92_RS11955	2555193..2557421	+	2229	WP_003099265.1	TonB-dependent receptor	-
A4W92_RS11960	2557491..2558138	-	648	WP_003095021.1	carbonate dehydratase	-
A4W92_RS11965	2558200..2559438	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T62382 WP_003117425.1 NZ_CP015117:c2554864-2554586 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T62382 NZ_CP015117:c2554864-2554586 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT62382 WP_003099268.1 NZ_CP015117:c2554574-2554269 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT62382 NZ_CP015117:c2554574-2554269 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5