Detailed information of TA system TA062377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 245022..245674
• Replicon: plasmid unnamed
• Accession: NZ_CP015116
• Organism: Ralstonia solanacearum strain EP1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A454TTN3
• Locus tag: AC251_RS19730
• Protein ID: WP_038962847.1
• Coordinates: 245022..245366 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AC251_RS19735
• Protein ID: WP_020425351.1
• Coordinates: 245363..245674 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC251_RS19715	240717..241880	-	1164	WP_011003438.1	2-methylcitrate synthase	-
AC251_RS19720	241932..242828	-	897	WP_081365370.1	methylisocitrate lyase	-
AC251_RS19725	243022..244971	+	1950	WP_023470006.1	propionate catabolism operon regulatory protein PrpR	-
AC251_RS19730	245022..245366	+	345	WP_038962847.1	hypothetical protein	Toxin
AC251_RS19735	245363..245674	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
AC251_RS19740	245814..246623	-	810	WP_043885575.1	DUF2242 domain-containing protein	-
AC251_RS19750	247868..249706	+	1839	WP_071653875.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / htpB / adeG / cheY / cheB / cheW / motA	1..2093441	2093441

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13227.94 Da
• Isoelectric Point: 8.4489

>T62377 WP_038962847.1 NZ_CP015116:245022-245366 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIDYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T62377 NZ_CP015116:245022-245366 [Ralstonia solanacearum]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAACAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTGACTACCACTGGGATGGAGGCGGTCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT62377 WP_020425351.1 NZ_CP015116:245363-245674 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT62377 NZ_CP015116:245363-245674 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A454TTN3