Detailed information of TA system TA062333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 9888..10449
• Replicon: chromosome
• Accession: NZ_CP015099
• Organism: Glaesserella parasuis strain SC1401

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143CE91
• Locus tag: A4U84_RS00035
• Protein ID: WP_005712614.1
• Coordinates: 9888..10166 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: A4U84_RS00040
• Protein ID: WP_005712616.1
• Coordinates: 10177..10449 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4U84_RS00030	8729..9739	-	1011	WP_038513972.1	ornithine carbamoyltransferase	-
A4U84_RS00035	9888..10166	+	279	WP_005712614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4U84_RS00040	10177..10449	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
A4U84_RS00045	10513..11733	+	1221	WP_021112303.1	ATP-dependent RNA helicase RhlB	-
A4U84_RS00050	11743..13230	+	1488	WP_062923800.1	DUF853 domain-containing protein	-
A4U84_RS00055	13391..13933	-	543	WP_021115958.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10326.71 Da
• Isoelectric Point: 10.0505

>T62333 WP_005712614.1 NZ_CP015099:9888-10166 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T62333 NZ_CP015099:9888-10166 [Glaesserella parasuis]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACTGCAGGTATTCAGGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ATCAATTACAAGGTAATCTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAATTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT62333 WP_005712616.1 NZ_CP015099:10177-10449 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT62333 NZ_CP015099:10177-10449 [Glaesserella parasuis]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGTGTCACTCGTGTCACGCTTTCTCGTATTTTAAATGGCAAAGCTGCAATTTCACCTGAAATGGCTCTTAGAT
TAAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: A0A143CE91