Detailed information of TA system TA062323

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 95790..96029
• Replicon: plasmid unnamed
• Accession: NZ_CP015086
• Organism: Escherichia coli O25b:H4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: WLH_RS28410
• Protein ID: WP_023144756.1
• Coordinates: 95895..96029 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	95790..95850 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WLH_RS28385	93320..94066	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
WLH_RS28390	94121..94681	+	561	WP_000139341.1	fertility inhibition protein FinO	-
WLH_RS28395	94812..95024	+	213	WP_013023861.1	hypothetical protein	-
WLH_RS28405	95537..95823	+	287	Protein_105	DUF2726 domain-containing protein	-
-	95790..95850	-	61	NuclAT_2	-	Antitoxin
-	95790..95850	-	61	NuclAT_2	-	Antitoxin
-	95790..95850	-	61	NuclAT_2	-	Antitoxin
-	95790..95850	-	61	NuclAT_2	-	Antitoxin
WLH_RS28410	95895..96029	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
WLH_RS29100	96326..96400	+	75	WP_108401337.1	hypothetical protein	-
WLH_RS28415	96458..97204	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
WLH_RS28420	97219..98508	-	1290	Protein_109	IS21-like element ISEc12 family transposase	-
WLH_RS28425	98562..99266	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
WLH_RS28430	99332..99844	+	513	Protein_111	Tn3 family transposase	-
WLH_RS28435	99868..100473	-	606	WP_000509965.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	-	1..130920	130920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T62323 WP_023144756.1 NZ_CP015086:95895-96029 [Escherichia coli O25b:H4]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T62323 NZ_CP015086:95895-96029 [Escherichia coli O25b:H4]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT62323 NZ_CP015086:c95850-95790 [Escherichia coli O25b:H4]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin