Detailed information of TA system TA062319

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67571..67841
• Replicon: plasmid unnamed
• Accession: NZ_CP015086
• Organism: Escherichia coli O25b:H4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: WLH_RS28235
• Protein ID: WP_001312861.1
• Coordinates: 67683..67841 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67571..67634 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WLH_RS28210	62793..64751	+	1959	WP_042045562.1	ParB/RepB/Spo0J family partition protein	-
WLH_RS28215	64806..65240	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
WLH_RS28220	65237..65956	+	720	WP_033550421.1	plasmid SOS inhibition protein A	-
-	65968..66070	+	103	NuclAT_1	-	-
-	65968..66070	+	103	NuclAT_1	-	-
-	65968..66070	+	103	NuclAT_1	-	-
-	65968..66070	+	103	NuclAT_1	-	-
WLH_RS28225	66116..67485	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	67512..67639	+	128	NuclAT_0	-	-
-	67512..67639	+	128	NuclAT_0	-	-
-	67512..67639	+	128	NuclAT_0	-	-
-	67512..67639	+	128	NuclAT_0	-	-
-	67571..67634	-	64	-	-	Antitoxin
WLH_RS28235	67683..67841	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
WLH_RS29580	68284..68619	+	336	WP_013023876.1	hypothetical protein	-
WLH_RS29585	68532..68738	+	207	WP_000275859.1	hypothetical protein	-
WLH_RS28255	68763..69050	+	288	WP_000107535.1	hypothetical protein	-
WLH_RS28260	69169..69990	+	822	WP_001234469.1	DUF945 domain-containing protein	-
WLH_RS28265	70287..70889	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
WLH_RS28270	71219..71602	+	384	WP_001151566.1	relaxosome protein TraM	-
WLH_RS28275	71736..72413	+	678	WP_001348626.1	PAS domain-containing protein	-
WLH_RS28280	72501..72728	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	-	1..130920	130920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T62319 WP_001312861.1 NZ_CP015086:67683-67841 [Escherichia coli O25b:H4]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T62319 NZ_CP015086:67683-67841 [Escherichia coli O25b:H4]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT62319 NZ_CP015086:c67634-67571 [Escherichia coli O25b:H4]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin