62313

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4299547..4300226 Replicon chromosome
Accession NZ_CP015085
Organism Escherichia coli O25b:H4

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag WLH_RS22275 Protein ID WP_000057523.1
Coordinates 4299547..4299849 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag WLH_RS22280 Protein ID WP_000806442.1
Coordinates 4299885..4300226 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WLH_RS22245 4294923..4296575 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
WLH_RS22250 4296613..4297116 - 504 WP_000667000.1 hypothetical protein -
WLH_RS22255 4297113..4297913 - 801 WP_000439798.1 hypothetical protein -
WLH_RS22260 4297937..4298416 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
WLH_RS22270 4298620..4299414 - 795 WP_000365147.1 TraB/GumN family protein -
WLH_RS22275 4299547..4299849 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
WLH_RS22280 4299885..4300226 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
WLH_RS22285 4300284..4302788 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
WLH_RS22290 4303050..4303982 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T62313 WP_000057523.1 NZ_CP015085:4299547-4299849 [Escherichia coli O25b:H4]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T62313 NZ_CP015085:4299547-4299849 [Escherichia coli O25b:H4]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT62313 WP_000806442.1 NZ_CP015085:4299885-4300226 [Escherichia coli O25b:H4]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT62313 NZ_CP015085:4299885-4300226 [Escherichia coli O25b:H4]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
AlphaFold DB S1QAY3

References