Detailed information of TA system TA062302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2984764..2985366
• Replicon: chromosome
• Accession: NZ_CP015085
• Organism: Escherichia coli O25b:H4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: WLH_RS15470
• Protein ID: WP_000897302.1
• Coordinates: 2984764..2985075 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WLH_RS15475
• Protein ID: WP_000356397.1
• Coordinates: 2985076..2985366 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WLH_RS15445	2980678..2981277	+	600	WP_001296610.1	glucose-1-phosphatase	-
WLH_RS15450	2981271..2982143	+	873	WP_000920762.1	virulence factor BrkB family protein	-
WLH_RS15455	2982140..2982577	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
WLH_RS15460	2982622..2983563	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
WLH_RS15465	2983627..2984535	-	909	WP_001385591.1	alpha/beta hydrolase	-
WLH_RS15470	2984764..2985075	+	312	WP_000897302.1	hypothetical protein	Toxin
WLH_RS15475	2985076..2985366	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
WLH_RS15485	2985725..2986003	+	279	WP_001296612.1	hypothetical protein	-
WLH_RS15495	2986400..2986618	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
WLH_RS15500	2986803..2987252	-	450	WP_032140890.1	hypothetical protein	-
WLH_RS15505	2987568..2988416	-	849	WP_001038650.1	hypothetical protein	-
WLH_RS15510	2988706..2988948	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
WLH_RS15515	2989130..2990059	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T62302 WP_000897302.1 NZ_CP015085:2984764-2985075 [Escherichia coli O25b:H4]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T62302 NZ_CP015085:2984764-2985075 [Escherichia coli O25b:H4]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT62302 WP_000356397.1 NZ_CP015085:2985076-2985366 [Escherichia coli O25b:H4]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT62302 NZ_CP015085:2985076-2985366 [Escherichia coli O25b:H4]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416