Detailed information of TA system TA062296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 811869..812371
• Replicon: chromosome
• Accession: NZ_CP015085
• Organism: Escherichia coli O25b:H4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: WLH_RS04550
• Protein ID: WP_000767819.1
• Coordinates: 811869..812123 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: WLH_RS04555
• Protein ID: WP_001259253.1
• Coordinates: 812120..812371 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WLH_RS04520	807687..808145	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
WLH_RS04530	808362..809720	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
WLH_RS04535	809710..809772	-	63	WP_010723108.1	membrane protein YoeI	-
WLH_RS04540	809987..810916	-	930	WP_000803366.1	LysR family transcriptional regulator	-
WLH_RS04545	810962..811786	-	825	WP_000754737.1	SDR family oxidoreductase	-
WLH_RS04550	811869..812123	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
WLH_RS04555	812120..812371	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
WLH_RS04565	812654..812704	+	51	WP_001364200.1	his operon leader peptide	-
WLH_RS04575	812850..813749	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
WLH_RS04580	813755..815059	+	1305	WP_001296211.1	histidinol dehydrogenase	-
WLH_RS04585	815056..816126	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
WLH_RS04590	816126..817193	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	807687..808145	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T62296 WP_000767819.1 NZ_CP015085:c812123-811869 [Escherichia coli O25b:H4]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T62296 NZ_CP015085:c812123-811869 [Escherichia coli O25b:H4]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT62296 WP_001259253.1 NZ_CP015085:c812371-812120 [Escherichia coli O25b:H4]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT62296 NZ_CP015085:c812371-812120 [Escherichia coli O25b:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3