Detailed information of TA system TA062278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1840444..1841048
• Replicon: chromosome
• Accession: NZ_CP015080
• Organism: Desulfuromonas sp. DDH964

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143B7E5
• Locus tag: DBW_RS08305
• Protein ID: WP_066726816.1
• Coordinates: 1840444..1840764 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A143B763
• Locus tag: DBW_RS08310
• Protein ID: WP_066726818.1
• Coordinates: 1840767..1841048 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBW_RS08280	1835931..1837142	+	1212	WP_066726806.1	cofactor-independent phosphoglycerate mutase	-
DBW_RS08285	1837196..1837789	+	594	WP_066726808.1	2-oxoacid:acceptor oxidoreductase family protein	-
DBW_RS08290	1837782..1838090	+	309	WP_066726810.1	4Fe-4S binding protein	-
DBW_RS08295	1838087..1839298	+	1212	WP_066726812.1	pyruvate ferredoxin oxidoreductase	-
DBW_RS08300	1839295..1840206	+	912	WP_066726814.1	2-ketoisovalerate ferredoxin oxidoreductase	-
DBW_RS08305	1840444..1840764	+	321	WP_066726816.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBW_RS08310	1840767..1841048	+	282	WP_066726818.1	helix-turn-helix domain-containing protein	Antitoxin
DBW_RS08315	1841259..1841942	+	684	WP_157471843.1	IS3 family transposase	-
DBW_RS08320	1842187..1842471	+	285	WP_066726820.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
DBW_RS08325	1842478..1842777	+	300	WP_066726822.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DBW_RS08330	1843228..1844247	-	1020	WP_066722476.1	IS110 family transposase	-
DBW_RS08335	1844459..1844806	+	348	WP_066729743.1	DUF488 domain-containing protein	-
DBW_RS08340	1844952..1845605	-	654	WP_082820256.1	glycine zipper 2TM domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1841319..1841942	623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12418.69 Da
• Isoelectric Point: 10.2633

>T62278 WP_066726816.1 NZ_CP015080:1840444-1840764 [Desulfuromonas sp. DDH964]
MRWTVEFLNEVVEEEFRELPKDMQARFVRISELIEGCGIEQVGMPHVRHLEGKLWEMRARGIAGISRGIYVAINGRRVVI
LRIFVKKTQKTPRSEILLALDRMKEL

Nucleotide

• Length: 321 bp

>T62278 NZ_CP015080:1840444-1840764 [Desulfuromonas sp. DDH964]
ATGCGTTGGACCGTTGAATTTCTGAACGAGGTTGTTGAAGAAGAATTCCGTGAATTGCCCAAGGATATGCAGGCTCGGTT
CGTGCGTATCTCCGAACTGATCGAAGGTTGCGGAATCGAGCAGGTGGGAATGCCTCATGTGCGCCACCTTGAAGGTAAGC
TTTGGGAGATGCGGGCGAGGGGAATCGCTGGAATCTCCCGGGGGATTTATGTTGCGATAAACGGCAGGCGCGTGGTGATC
CTGCGAATCTTCGTGAAGAAGACCCAGAAGACGCCGCGATCGGAAATTCTGCTGGCGCTGGACCGGATGAAGGAGTTGTG
A

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10681.34 Da
• Isoelectric Point: 10.3074

>AT62278 WP_066726818.1 NZ_CP015080:1840767-1841048 [Desulfuromonas sp. DDH964]
MGKKIRDLKEELLANEEFRREYQPLDEEFSIAAQLIEARTKANLTQEQVARRMGTTQSVVARLESGHPMPSLRSLRRYAL
AVGNKVEIKLVQK

Nucleotide

• Length: 282 bp

>AT62278 NZ_CP015080:1840767-1841048 [Desulfuromonas sp. DDH964]
ATGGGGAAAAAGATCAGAGATTTGAAGGAAGAGTTGTTGGCCAACGAGGAATTCCGCCGTGAGTATCAGCCGCTGGACGA
GGAATTCTCCATCGCTGCACAACTGATTGAAGCTAGAACCAAGGCAAACCTCACACAGGAACAAGTGGCCAGACGCATGG
GTACTACCCAATCGGTCGTGGCACGGCTCGAGTCCGGTCATCCGATGCCGAGTTTGCGGTCCTTGCGACGGTATGCGTTG
GCGGTTGGGAACAAGGTGGAGATCAAGTTGGTTCAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A143B7E5

Antitoxin

• AlphaFold DB: A0A143B763