Detailed information of TA system TA062264

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5284..5613
• Replicon: plasmid pEC448_1
• Accession: NZ_CP015077
• Organism: Escherichia coli strain Ecol_448

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A4R37_RS24940
• Protein ID: WP_001312861.1
• Coordinates: 5284..5442 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	5486..5613 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R37_RS24900	397..624	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
A4R37_RS24905	712..1389	-	678	WP_001348626.1	PAS domain-containing protein	-
A4R37_RS24910	1523..1906	-	384	WP_001151566.1	relaxosome protein TraM	-
A4R37_RS24915	2237..2839	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
A4R37_RS24920	3136..3957	-	822	WP_001234469.1	DUF945 domain-containing protein	-
A4R37_RS24925	4075..4362	-	288	WP_000107535.1	hypothetical protein	-
A4R37_RS29800	4387..4593	-	207	WP_000275859.1	hypothetical protein	-
A4R37_RS29805	4506..4841	-	336	WP_013023876.1	hypothetical protein	-
A4R37_RS24940	5284..5442	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	5486..5613	-	128	NuclAT_0	-	Antitoxin
-	5486..5613	-	128	NuclAT_0	-	Antitoxin
-	5486..5613	-	128	NuclAT_0	-	Antitoxin
-	5486..5613	-	128	NuclAT_0	-	Antitoxin
-	7055..7157	-	103	NuclAT_1	-	-
-	7055..7157	-	103	NuclAT_1	-	-
-	7055..7157	-	103	NuclAT_1	-	-
-	7055..7157	-	103	NuclAT_1	-	-
A4R37_RS24960	7169..7888	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
A4R37_RS24965	7885..8319	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
A4R37_RS24970	8374..10332	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..133735	133735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T62264 WP_001312861.1 NZ_CP015077:c5442-5284 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T62264 NZ_CP015077:c5442-5284 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 128 bp

>AT62264 NZ_CP015077:c5613-5486 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin