62249

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1702248..1702750 Replicon chromosome
Accession NZ_CP015076
Organism Escherichia coli strain Ecol_448

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag A4R37_RS09010 Protein ID WP_000767822.1
Coordinates 1702248..1702502 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A4R37_RS09015 Protein ID WP_001259255.1
Coordinates 1702499..1702750 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R37_RS08995 1698741..1700099 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
A4R37_RS29055 1700089..1700151 - 63 WP_010723108.1 membrane protein YoeI -
A4R37_RS09000 1700366..1701295 - 930 WP_000803366.1 LysR family transcriptional regulator -
A4R37_RS09005 1701341..1702165 - 825 WP_000754737.1 SDR family oxidoreductase -
A4R37_RS09010 1702248..1702502 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A4R37_RS09015 1702499..1702750 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A4R37_RS26720 1703033..1703083 + 51 WP_001364200.1 his operon leader peptide -
A4R37_RS09020 1703229..1704128 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
A4R37_RS09025 1704134..1705438 + 1305 WP_001546022.1 histidinol dehydrogenase -
A4R37_RS09030 1705435..1706505 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
A4R37_RS09035 1706505..1707572 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T62249 WP_000767822.1 NZ_CP015076:c1702502-1702248 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T62249 NZ_CP015076:c1702502-1702248 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT62249 WP_001259255.1 NZ_CP015076:c1702750-1702499 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT62249 NZ_CP015076:c1702750-1702499 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References