Detailed information of TA system TA062204

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50899..51325
• Replicon: plasmid pEC743_3
• Accession: NZ_CP015072
• Organism: Escherichia coli strain Ecol_743

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A4R39_RS27485
• Protein ID: WP_001312861.1
• Coordinates: 50899..51057 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51101..51325 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R39_RS25195	45922..46149	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
A4R39_RS25200	46286..46957	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
A4R39_RS25205	47151..47534	-	384	WP_001354030.1	relaxosome protein TraM	-
A4R39_RS25210	47857..48459	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
A4R39_RS25215	48756..49577	-	822	WP_001234445.1	DUF945 domain-containing protein	-
A4R39_RS25220	49688..49984	-	297	WP_001272251.1	hypothetical protein	-
A4R39_RS28975	50284..50661	+	378	Protein_62	hypothetical protein	-
A4R39_RS27485	50899..51057	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	51101..51325	-	225	NuclAT_0	-	Antitoxin
-	51101..51325	-	225	NuclAT_0	-	Antitoxin
-	51101..51325	-	225	NuclAT_0	-	Antitoxin
-	51101..51325	-	225	NuclAT_0	-	Antitoxin
A4R39_RS28890	51137..51316	+	180	WP_001309233.1	hypothetical protein	-
A4R39_RS25235	51337..52056	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
A4R39_RS25240	52053..52487	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
A4R39_RS25245	52556..54520	-	1965	WP_063131970.1	ParB/RepB/Spo0J family partition protein	-
A4R39_RS25250	54581..54814	-	234	WP_000006003.1	DUF905 family protein	-
A4R39_RS25255	54872..55357	-	486	WP_031311812.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..65196	65196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T62204 WP_001312861.1 NZ_CP015072:c51057-50899 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T62204 NZ_CP015072:c51057-50899 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT62204 NZ_CP015072:c51325-51101 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin