Detailed information of TA system TA062200

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83167..83409
• Replicon: plasmid pEC743_1
• Accession: NZ_CP015070
• Organism: Escherichia coli strain Ecol_743

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A4R39_RS24320
• Protein ID: WP_001312861.1
• Coordinates: 83167..83325 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83369..83409 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R39_RS24285	78279..78506	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
A4R39_RS24290	78594..79271	-	678	WP_001348626.1	PAS domain-containing protein	-
A4R39_RS24295	79405..79788	-	384	WP_001151566.1	relaxosome protein TraM	-
A4R39_RS24300	80119..80721	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
A4R39_RS24305	81018..81839	-	822	WP_001234469.1	DUF945 domain-containing protein	-
A4R39_RS24310	81958..82245	-	288	WP_000107535.1	hypothetical protein	-
A4R39_RS28110	82270..82476	-	207	WP_000275859.1	hypothetical protein	-
A4R39_RS28970	82389..82724	-	336	WP_013023876.1	hypothetical protein	-
A4R39_RS24320	83167..83325	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	83369..83409	-	41	NuclAT_1	-	Antitoxin
-	83369..83409	-	41	NuclAT_1	-	Antitoxin
-	83369..83409	-	41	NuclAT_1	-	Antitoxin
-	83369..83409	-	41	NuclAT_1	-	Antitoxin
-	84853..85039	-	187	NuclAT_0	-	-
-	84853..85039	-	187	NuclAT_0	-	-
-	84853..85039	-	187	NuclAT_0	-	-
-	84853..85039	-	187	NuclAT_0	-	-
A4R39_RS24340	85051..85770	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
A4R39_RS24345	85767..86201	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
A4R39_RS24350	86256..88214	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..111851	111851

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T62200 WP_001312861.1 NZ_CP015070:c83325-83167 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T62200 NZ_CP015070:c83325-83167 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT62200 NZ_CP015070:c83409-83369 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin