Detailed information of TA system TA062196

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41339..41578
• Replicon: plasmid pEC743_1
• Accession: NZ_CP015070
• Organism: Escherichia coli strain Ecol_743

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: A4R39_RS24095
• Protein ID: WP_023144756.1
• Coordinates: 41339..41473 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41518..41578 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R39_RS24065	36404..37101	-	698	Protein_37	IS1 family transposase	-
A4R39_RS24070	37350..37751	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
A4R39_RS28700	37684..37941	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
A4R39_RS24080	38034..38687	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
A4R39_RS24085	39627..40484	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
A4R39_RS27340	40477..40551	-	75	WP_031943482.1	RepA leader peptide Tap	-
A4R39_RS28965	40548..40682	-	135	Protein_43	protein CopA/IncA	-
A4R39_RS24090	40788..41042	-	255	WP_000083850.1	replication regulatory protein RepA	-
A4R39_RS24095	41339..41473	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	41518..41578	+	61	NuclAT_2	-	Antitoxin
-	41518..41578	+	61	NuclAT_2	-	Antitoxin
-	41518..41578	+	61	NuclAT_2	-	Antitoxin
-	41518..41578	+	61	NuclAT_2	-	Antitoxin
A4R39_RS27350	41545..41831	-	287	Protein_46	DUF2726 domain-containing protein	-
A4R39_RS24100	41909..43522	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
A4R39_RS24105	43553..43903	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
A4R39_RS24110	43900..44325	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
A4R39_RS24115	44884..45096	-	213	WP_013023861.1	hypothetical protein	-
A4R39_RS24120	45227..45787	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..111851	111851

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T62196 WP_023144756.1 NZ_CP015070:c41473-41339 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T62196 NZ_CP015070:c41473-41339 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT62196 NZ_CP015070:41518-41578 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin