Detailed information of TA system TA062156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2776940..2777462
• Replicon: chromosome
• Accession: NZ_CP015064
• Organism: Mesorhizobium ciceri biovar biserrulae strain WSM1284

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: A4R29_RS12770
• Protein ID: WP_063172049.1
• Coordinates: 2776940..2777125 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A807AKQ0
• Locus tag: A4R29_RS12775
• Protein ID: WP_063172050.1
• Coordinates: 2777163..2777462 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R29_RS12750	2772161..2772907	+	747	WP_063173667.1	cytochrome c biogenesis CcdA family protein	-
A4R29_RS12755	2772990..2773427	+	438	WP_013531913.1	hypothetical protein	-
A4R29_RS12760	2773575..2774933	+	1359	WP_063172047.1	bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU	-
A4R29_RS12765	2775106..2776929	+	1824	WP_063172048.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
A4R29_RS12770	2776940..2777125	+	186	WP_063172049.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4R29_RS12775	2777163..2777462	+	300	WP_063172050.1	HigA family addiction module antidote protein	Antitoxin
A4R29_RS12780	2777543..2778298	+	756	WP_024503567.1	DUF502 domain-containing protein	-
A4R29_RS12785	2778250..2780358	-	2109	WP_063172051.1	ATP-dependent DNA helicase RecG	-
A4R29_RS12790	2780451..2780738	+	288	WP_013531907.1	succinate dehydrogenase assembly factor 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-61)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6794.59 Da
• Isoelectric Point: 6.7112

>T62156 WP_063172049.1 NZ_CP015064:2776940-2777125 [Mesorhizobium ciceri biovar biserrulae]
ILRAIANAAQLNDLRSPPGNHLEALNGSRAGQHSIRINDQWRICFKWTAAGAEDVEIVDYH

Nucleotide

• Length: 186 bp

>T62156 NZ_CP015064:2776940-2777125 [Mesorhizobium ciceri biovar biserrulae]
ATCTTGCGCGCAATTGCCAATGCTGCCCAACTGAATGACCTTCGATCGCCGCCCGGAAATCATCTTGAAGCCCTGAACGG
GAGCCGAGCCGGACAGCATTCAATTCGCATCAATGACCAGTGGCGCATCTGCTTCAAATGGACCGCTGCTGGCGCTGAAG
ACGTGGAGATCGTCGATTACCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11271.11 Da
• Isoelectric Point: 5.2204

>AT62156 WP_063172050.1 NZ_CP015064:2777163-2777462 [Mesorhizobium ciceri biovar biserrulae]
MLKFARPVHPGEFLREEYLVPLGMSAGALAKKLNLPRTGIERIAKEEIGLTPDTALRLARFFNTTPEFWMNFQQAYELET
EARKLAPELEKIEPLEVAA

Nucleotide

• Length: 300 bp

>AT62156 NZ_CP015064:2777163-2777462 [Mesorhizobium ciceri biovar biserrulae]
ATGCTCAAGTTCGCACGTCCAGTTCATCCTGGAGAATTCCTGCGTGAAGAATATCTCGTCCCGCTCGGCATGAGTGCAGG
CGCGCTGGCGAAAAAGCTCAACCTTCCGCGCACCGGTATCGAACGCATTGCCAAAGAAGAGATCGGCCTGACTCCCGATA
CCGCTCTTCGCCTCGCCAGATTTTTCAACACCACGCCCGAATTCTGGATGAATTTTCAACAGGCCTATGAGCTTGAAACC
GAAGCCAGAAAGCTGGCGCCGGAGCTCGAGAAGATTGAGCCGTTGGAAGTAGCGGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A807AKQ0