Detailed information of TA system TA062129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 52909..53636
• Replicon: plasmid pKPN-065
• Accession: NZ_CP015026
• Organism: Klebsiella pneumoniae strain Kpn223

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: WM91_RS26965
• Protein ID: WP_011251285.1
• Coordinates: 52909..53220 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WM91_RS26970
• Protein ID: WP_011251286.1
• Coordinates: 53217..53636 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM91_RS29705	49675..49854	-	180	Protein_48	IS5/IS1182 family transposase	-
WM91_RS26940	49894..51422	-	1529	Protein_49	IS66 family transposase	-
WM91_RS26950	51471..51818	-	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
WM91_RS26955	51815..52153	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
WM91_RS26960	52246..52704	+	459	Protein_52	DDE-type integrase/transposase/recombinase	-
WM91_RS26965	52909..53220	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
WM91_RS26970	53217..53636	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
WM91_RS26975	53783..54751	+	969	WP_077268729.1	IS5 family transposase	-
WM91_RS29135	54820..55355	-	536	Protein_56	transposase	-
WM91_RS26990	55880..57247	-	1368	WP_032437385.1	formimidoylglutamate deiminase	-
WM91_RS26995	57350..58111	+	762	WP_017900871.1	histidine utilization repressor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / sul2	-	1..170926	170926
	-	inside	IScluster/Tn	-	-	49675..54751	5076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T62129 WP_011251285.1 NZ_CP015026:52909-53220 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T62129 NZ_CP015026:52909-53220 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT62129 WP_011251286.1 NZ_CP015026:53217-53636 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT62129 NZ_CP015026:53217-53636 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1