Detailed information of TA system TA062100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3834190..3834884
• Replicon: chromosome
• Accession: NZ_CP015023
• Organism: Escherichia coli strain SRCC 1675

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: AR439_RS19935
• Protein ID: WP_001263495.1
• Coordinates: 3834486..3834884 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: AR439_RS19930
• Protein ID: WP_000554757.1
• Coordinates: 3834190..3834483 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AR439_RS32895	3829901..3830398	+	498	Protein_3655	REP-associated tyrosine transposase RayT	-
AR439_RS19915	3830543..3832255	-	1713	Protein_3656	flagellar biosynthesis protein FlhA	-
AR439_RS19920	3832227..3833012	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AR439_RS19925	3833083..3834138	+	1056	WP_001226188.1	DNA polymerase IV	-
AR439_RS19930	3834190..3834483	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AR439_RS19935	3834486..3834884	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AR439_RS19940	3834894..3835346	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
AR439_RS19945	3835665..3835868	+	204	Protein_3662	RNA ligase RtcB family protein	-
AR439_RS19950	3835867..3836388	+	522	Protein_3663	peptide chain release factor H	-
AR439_RS19955	3836445..3837902	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
AR439_RS19960	3838163..3838621	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	3839217..3839297	+	81	NuclAT_12	-	-
-	3839217..3839297	+	81	NuclAT_12	-	-
-	3839217..3839297	+	81	NuclAT_12	-	-
-	3839217..3839297	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T62100 WP_001263495.1 NZ_CP015023:3834486-3834884 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T62100 NZ_CP015023:3834486-3834884 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT62100 WP_000554757.1 NZ_CP015023:3834190-3834483 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT62100 NZ_CP015023:3834190-3834483 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6