Detailed information of TA system TA062066

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4942238..4942463
• Replicon: chromosome
• Accession: NZ_CP015020
• Organism: Escherichia coli strain 28RC1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ARC77_RS24940
• Protein ID: WP_000813263.1
• Coordinates: 4942308..4942463 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4942238..4942296 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ARC77_RS24915	4937519..4938604	+	1086	WP_072141648.1	DNA-binding protein	-
ARC77_RS24920	4938611..4939357	+	747	WP_000788745.1	ATP-binding protein	-
ARC77_RS24925	4939379..4940149	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
ARC77_RS24930	4940165..4940578	+	414	WP_001151233.1	DUF977 family protein	-
ARC77_RS24935	4940930..4941703	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	4942238..4942296	-	59	-	-	Antitoxin
ARC77_RS24940	4942308..4942463	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
ARC77_RS32080	4942631..4942909	+	279	WP_001341388.1	hypothetical protein	-
ARC77_RS24950	4942911..4943960	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
ARC77_RS24955	4943973..4944344	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ARC77_RS24960	4944334..4944705	+	372	WP_000090264.1	antitermination protein	-
ARC77_RS24965	4944857..4945675	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ARC77_RS24970	4945962..4946158	+	197	Protein_4711	TrmB family transcriptional regulator	-
ARC77_RS24975	4946296..4947009	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T62066 WP_000813263.1 NZ_CP015020:4942308-4942463 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T62066 NZ_CP015020:4942308-4942463 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT62066 NZ_CP015020:c4942296-4942238 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin