Detailed information of TA system TA062062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4055110..4055804
• Replicon: chromosome
• Accession: NZ_CP015020
• Organism: Escherichia coli strain 28RC1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ARC77_RS20670
• Protein ID: WP_001263495.1
• Coordinates: 4055406..4055804 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ARC77_RS20665
• Protein ID: WP_000554757.1
• Coordinates: 4055110..4055403 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ARC77_RS32920	4050821..4051318	+	498	Protein_3891	REP-associated tyrosine transposase RayT	-
ARC77_RS20650	4051463..4053175	-	1713	Protein_3892	flagellar biosynthesis protein FlhA	-
ARC77_RS20655	4053147..4053932	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ARC77_RS20660	4054003..4055058	+	1056	WP_001226186.1	DNA polymerase IV	-
ARC77_RS20665	4055110..4055403	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ARC77_RS20670	4055406..4055804	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ARC77_RS20675	4055814..4056266	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ARC77_RS20680	4056585..4056788	+	204	Protein_3898	RNA ligase RtcB family protein	-
ARC77_RS20685	4056787..4057308	+	522	Protein_3899	peptide chain release factor H	-
ARC77_RS20690	4057365..4058822	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ARC77_RS20695	4059083..4059541	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	4060137..4060217	+	81	NuclAT_11	-	-
-	4060137..4060217	+	81	NuclAT_11	-	-
-	4060137..4060217	+	81	NuclAT_11	-	-
-	4060137..4060217	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4049872..4093964	44092

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T62062 WP_001263495.1 NZ_CP015020:4055406-4055804 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T62062 NZ_CP015020:4055406-4055804 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT62062 WP_000554757.1 NZ_CP015020:4055110-4055403 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT62062 NZ_CP015020:4055110-4055403 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6