Detailed information of TA system TA062056

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 3701445..3701857
• Replicon: chromosome
• Accession: NZ_CP015020
• Organism: Escherichia coli strain 28RC1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: ARC77_RS18990
• Protein ID: WP_000132630.1
• Coordinates: 3701445..3701786 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	3701781..3701857 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ARC77_RS33405	3697013..3697213	+	201	WP_001310455.1	hypothetical protein	-
ARC77_RS18970	3697456..3698868	-	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
ARC77_RS18975	3699045..3699209	+	165	WP_000394274.1	DUF1127 domain-containing protein	-
ARC77_RS18980	3699306..3700187	+	882	WP_001304007.1	DUF262 domain-containing protein	-
ARC77_RS30375	3700235..3701398	+	1164	WP_001304006.1	DUF1524 domain-containing protein	-
ARC77_RS18990	3701445..3701786	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
-	3701781..3701857	+	77	NuclAT_14	-	Antitoxin
-	3701781..3701857	+	77	NuclAT_14	-	Antitoxin
-	3701781..3701857	+	77	NuclAT_14	-	Antitoxin
-	3701781..3701857	+	77	NuclAT_14	-	Antitoxin
-	3701781..3701857	+	77	NuclAT_15	-	Antitoxin
-	3701781..3701857	+	77	NuclAT_15	-	Antitoxin
-	3701781..3701857	+	77	NuclAT_15	-	Antitoxin
-	3701781..3701857	+	77	NuclAT_15	-	Antitoxin
ARC77_RS18995	3702007..3703761	-	1755	WP_000110082.1	restriction endonuclease subunit S	-
ARC77_RS19000	3703761..3705230	-	1470	WP_001302468.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T62056 WP_000132630.1 NZ_CP015020:c3701786-3701445 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T62056 NZ_CP015020:c3701786-3701445 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT62056 NZ_CP015020:3701781-3701857 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin