Detailed information of TA system TA062042

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 737015..737240
• Replicon: chromosome
• Accession: NZ_CP015020
• Organism: Escherichia coli strain 28RC1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ARC77_RS03880
• Protein ID: WP_000813254.1
• Coordinates: 737015..737170 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	737182..737240 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ARC77_RS03850	732301..733359	-	1059	WP_000935552.1	site-specific DNA-methyltransferase	-
ARC77_RS03855	733510..733707	-	198	WP_000917735.1	hypothetical protein	-
ARC77_RS03860	733934..734755	-	822	WP_000762909.1	antitermination protein	-
ARC77_RS03865	734752..735126	-	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
ARC77_RS03870	735139..736188	-	1050	WP_001265167.1	DUF968 domain-containing protein	-
ARC77_RS31495	736190..736468	-	279	WP_032156506.1	hypothetical protein	-
ARC77_RS03875	736538..736798	-	261	WP_077625879.1	hypothetical protein	-
ARC77_RS03880	737015..737170	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	737182..737240	+	59	-	-	Antitoxin
ARC77_RS03885	737581..738375	+	795	WP_000373318.1	protein kinase	-
ARC77_RS03890	738359..739075	+	717	WP_000789359.1	protein phosphatase 2C domain-containing protein	-
ARC77_RS33310	739335..739508	-	174	WP_001224661.1	hypothetical protein	-
ARC77_RS03895	739603..739959	-	357	WP_000403785.1	hypothetical protein	-
ARC77_RS03900	740032..740412	-	381	WP_001118156.1	DUF977 family protein	-
ARC77_RS03905	740428..741198	-	771	WP_000450846.1	DUF1627 domain-containing protein	-
ARC77_RS32470	741232..741774	-	543	WP_157837342.1	replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC / stx2B / stx2A	704979..794733	89754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T62042 WP_000813254.1 NZ_CP015020:c737170-737015 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T62042 NZ_CP015020:c737170-737015 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT62042 NZ_CP015020:737182-737240 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin