Detailed information of TA system TA062039

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 377083..377308
• Replicon: chromosome
• Accession: NZ_CP015020
• Organism: Escherichia coli strain 28RC1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ARC77_RS02090
• Protein ID: WP_000813258.1
• Coordinates: 377153..377308 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	377083..377141 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ARC77_RS02040	372136..372378	+	243	WP_000747948.1	hypothetical protein	-
ARC77_RS02045	372362..372787	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ARC77_RS32385	372856..373911	+	1056	WP_001356791.1	hypothetical protein	-
ARC77_RS32390	373904..374365	+	462	WP_000139447.1	replication protein	-
ARC77_RS02060	374399..375115	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ARC77_RS02065	375148..375429	+	282	WP_000603384.1	DNA-binding protein	-
ARC77_RS02070	375426..375653	+	228	WP_000699809.1	hypothetical protein	-
ARC77_RS02075	375646..375957	+	312	WP_001289673.1	hypothetical protein	-
ARC77_RS02080	376085..376303	+	219	WP_000683609.1	DUF4014 family protein	-
ARC77_RS02085	376305..376862	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	377083..377141	-	59	-	-	Antitoxin
ARC77_RS02090	377153..377308	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ARC77_RS02095	377428..377772	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ARC77_RS02100	377894..378166	+	273	WP_000191871.1	hypothetical protein	-
ARC77_RS02105	378168..379217	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ARC77_RS02110	379230..379535	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ARC77_RS02115	379598..380152	+	555	WP_000640035.1	DUF1133 family protein	-
ARC77_RS32395	380377..380574	+	198	WP_000917763.1	hypothetical protein	-
ARC77_RS02125	380710..381423	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ARC77_RS02140	381874..382305	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	316408..416304	99896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T62039 WP_000813258.1 NZ_CP015020:377153-377308 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T62039 NZ_CP015020:377153-377308 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT62039 NZ_CP015020:c377141-377083 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin