Detailed information of TA system TA062001

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrE-as-bsrE/-
• Location: 2049429..2049744
• Replicon: chromosome
• Accession: NZ_CP015004
• Organism: Bacillus subtilis strain SZMC 6179J isolate B23

Toxin (Protein)

• Gene name: bsrE
• Uniprot ID: -
• Locus tag: A3772_RS22680
• Protein ID: WP_120322743.1
• Coordinates: 2049429..2049590 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	as-bsrE
Locus tag	-
Coordinates	2049571..2049744 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3772_RS10315	2044657..2044992	+	336	WP_009967409.1	hypothetical protein	-
A3772_RS10320	2045039..2048644	-	3606	WP_004399367.1	membrane protein	-
A3772_RS10325	2048877..2049209	-	333	WP_026009814.1	hypothetical protein	-
A3772_RS22680	2049429..2049590	+	162	WP_120322743.1	putative holin-like toxin	Toxin
-	2049571..2049744	-	174	-	-	Antitoxin
A3772_RS10330	2049859..2050701	-	843	WP_003231327.1	hypothetical protein	-
A3772_RS10335	2050901..2051359	-	459	WP_003231326.1	type II toxin-antitoxin system antitoxin YobK	-
A3772_RS10340	2051369..2053171	-	1803	WP_004399481.1	type II toxin-antitoxin system toxin ribonuclease YobL	-
A3772_RS10345	2053273..2053830	-	558	WP_004399321.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2035483..2060709	25226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (24-47)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5780.74 Da
• Isoelectric Point: 4.6641

>T62001 WP_120322743.1 NZ_CP015004:2049429-2049590 [Bacillus subtilis]
ISCTVSFAHSRESCSSPNERGDAMSTFQALMLMLAIGSFIIALLTYIEKIDLP

Nucleotide

• Length: 162 bp

>T62001 NZ_CP015004:2049429-2049590 [Bacillus subtilis]
ATTTCCTGTACAGTAAGTTTTGCTCACTCGAGGGAGTCTTGCTCATCCCCTAATGAAAGGGGTGATGCTATGTCAACATT
CCAGGCATTAATGCTAATGCTTGCAATCGGGTCGTTTATAATCGCCCTATTGACGTATATAGAAAAAATAGACCTCCCTT
GA

Antitoxin

Nucleotide

• Length: 174 bp

>AT62001 NZ_CP015004:c2049744-2049571 [Bacillus subtilis]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTC
TGTACAAAAAGTTGCCCATCAAAGGGCTTGCTCGATGTGGTTCTAGGTAGGCCTATCCCTTCAGGTTCTGAGGCTCAAGG
GAGGTCTATTTTTT

Structures

Antitoxin