Detailed information of TA system TA061996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5211016..5211697
• Replicon: chromosome
• Accession: NZ_CP015003
• Organism: Pseudomonas aeruginosa strain PA11803

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PA11803_RS24845
• Protein ID: WP_015503432.1
• Coordinates: 5211332..5211697 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA11803_RS24840
• Protein ID: WP_016561576.1
• Coordinates: 5211016..5211339 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA11803_RS24815	5206637..5207515	+	879	WP_003109435.1	hypothetical protein	-
PA11803_RS34715	5208027..5208893	-	867	WP_023081971.1	hypothetical protein	-
PA11803_RS34720	5208923..5209174	-	252	WP_124136459.1	hypothetical protein	-
PA11803_RS34220	5209391..5209945	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
PA11803_RS34225	5210119..5210394	-	276	WP_004353146.1	hypothetical protein	-
PA11803_RS24830	5210394..5210726	-	333	WP_016561578.1	hypothetical protein	-
PA11803_RS34725	5210781..5210900	+	120	Protein_4967	IS5/IS1182 family transposase	-
PA11803_RS24840	5211016..5211339	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
PA11803_RS24845	5211332..5211697	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA11803_RS24850	5211961..5212203	-	243	WP_043884955.1	hypothetical protein	-
PA11803_RS24855	5212410..5212682	+	273	WP_003085667.1	hypothetical protein	-
PA11803_RS24860	5212701..5213126	-	426	WP_003163196.1	VOC family protein	-
PA11803_RS24865	5213227..5214111	+	885	WP_019725847.1	LysR family transcriptional regulator	-
PA11803_RS24870	5214084..5215037	-	954	WP_003085661.1	LysR family transcriptional regulator	-
PA11803_RS24875	5215258..5215692	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T61996 WP_015503432.1 NZ_CP015003:c5211697-5211332 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T61996 NZ_CP015003:c5211697-5211332 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT61996 WP_016561576.1 NZ_CP015003:c5211339-5211016 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT61996 NZ_CP015003:c5211339-5211016 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA