Detailed information of TA system TA061991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5871563..5872158
• Replicon: chromosome
• Accession: NZ_CP015002
• Organism: Pseudomonas aeruginosa strain PA8281

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: PA8281_RS33385
• Protein ID: WP_003113526.1
• Coordinates: 5871880..5872158 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA8281_RS27490
• Protein ID: WP_003113527.1
• Coordinates: 5871563..5871868 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA8281_RS27455	5866704..5867552	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
PA8281_RS27465	5867719..5868660	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
PA8281_RS27470	5868777..5869391	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
PA8281_RS27475	5869433..5870017	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
PA8281_RS27480	5870058..5871158	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
PA8281_RS27490	5871563..5871868	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
PA8281_RS33385	5871880..5872158	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA8281_RS27495	5872487..5874715	+	2229	WP_003099265.1	TonB-dependent receptor	-
PA8281_RS27500	5874785..5875432	-	648	WP_003095021.1	carbonate dehydratase	-
PA8281_RS27505	5875494..5876732	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T61991 WP_003113526.1 NZ_CP015002:c5872158-5871880 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T61991 NZ_CP015002:c5872158-5871880 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT61991 WP_003113527.1 NZ_CP015002:c5871868-5871563 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT61991 NZ_CP015002:c5871868-5871563 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3