Detailed information of TA system TA061989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5012556..5013237
• Replicon: chromosome
• Accession: NZ_CP015002
• Organism: Pseudomonas aeruginosa strain PA8281

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PA8281_RS23390
• Protein ID: WP_015503432.1
• Coordinates: 5012872..5013237 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA8281_RS23385
• Protein ID: WP_016561576.1
• Coordinates: 5012556..5012879 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA8281_RS23360	5008177..5009055	+	879	WP_003109435.1	hypothetical protein	-
PA8281_RS33735	5009567..5010433	-	867	WP_023081971.1	hypothetical protein	-
PA8281_RS33740	5010463..5010714	-	252	WP_124136459.1	hypothetical protein	-
PA8281_RS33255	5010931..5011485	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
PA8281_RS33260	5011659..5011934	-	276	WP_004353146.1	hypothetical protein	-
PA8281_RS23375	5011934..5012266	-	333	WP_016561578.1	hypothetical protein	-
PA8281_RS33745	5012321..5012440	+	120	Protein_4665	IS5/IS1182 family transposase	-
PA8281_RS23385	5012556..5012879	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
PA8281_RS23390	5012872..5013237	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA8281_RS23395	5013501..5013743	-	243	WP_043884955.1	hypothetical protein	-
PA8281_RS23400	5013950..5014222	+	273	WP_003085667.1	hypothetical protein	-
PA8281_RS23405	5014241..5014666	-	426	WP_003163196.1	VOC family protein	-
PA8281_RS23410	5014767..5015651	+	885	WP_019725847.1	LysR family transcriptional regulator	-
PA8281_RS23415	5015624..5016577	-	954	WP_003085661.1	LysR family transcriptional regulator	-
PA8281_RS23420	5016798..5017232	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T61989 WP_015503432.1 NZ_CP015002:c5013237-5012872 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T61989 NZ_CP015002:c5013237-5012872 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT61989 WP_016561576.1 NZ_CP015002:c5012879-5012556 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT61989 NZ_CP015002:c5012879-5012556 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA