Detailed information of TA system TA061983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5679172..5679767
• Replicon: chromosome
• Accession: NZ_CP015001
• Organism: Pseudomonas aeruginosa strain PA1088

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: PA1088_RS32225
• Protein ID: WP_003113526.1
• Coordinates: 5679489..5679767 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA1088_RS26460
• Protein ID: WP_003113527.1
• Coordinates: 5679172..5679477 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA1088_RS26425	5674313..5675161	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
PA1088_RS26435	5675328..5676269	+	942	WP_070411033.1	ribose-phosphate pyrophosphokinase	-
PA1088_RS26440	5676386..5677000	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
PA1088_RS26445	5677042..5677626	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
PA1088_RS26450	5677667..5678767	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
PA1088_RS26460	5679172..5679477	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
PA1088_RS32225	5679489..5679767	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA1088_RS26465	5680096..5682324	+	2229	WP_003099265.1	TonB-dependent receptor	-
PA1088_RS26470	5682394..5683041	-	648	WP_003095021.1	carbonate dehydratase	-
PA1088_RS26475	5683103..5684341	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T61983 WP_003113526.1 NZ_CP015001:c5679767-5679489 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T61983 NZ_CP015001:c5679767-5679489 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT61983 WP_003113527.1 NZ_CP015001:c5679477-5679172 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT61983 NZ_CP015001:c5679477-5679172 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3