Detailed information of TA system TA061981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4914519..4915200
• Replicon: chromosome
• Accession: NZ_CP015001
• Organism: Pseudomonas aeruginosa strain PA1088

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PA1088_RS22775
• Protein ID: WP_015503432.1
• Coordinates: 4914835..4915200 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA1088_RS22770
• Protein ID: WP_016561576.1
• Coordinates: 4914519..4914842 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA1088_RS22745	4910140..4911018	+	879	WP_003109435.1	hypothetical protein	-
PA1088_RS32540	4911530..4912396	-	867	WP_023081971.1	hypothetical protein	-
PA1088_RS32545	4912426..4912677	-	252	WP_124136459.1	hypothetical protein	-
PA1088_RS32115	4912894..4913448	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
PA1088_RS32120	4913622..4913897	-	276	WP_004353146.1	hypothetical protein	-
PA1088_RS22760	4913897..4914229	-	333	WP_016561578.1	hypothetical protein	-
PA1088_RS32550	4914284..4914403	+	120	Protein_4533	IS5/IS1182 family transposase	-
PA1088_RS22770	4914519..4914842	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
PA1088_RS22775	4914835..4915200	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA1088_RS22780	4915464..4915706	-	243	WP_043884955.1	hypothetical protein	-
PA1088_RS22785	4915913..4916185	+	273	WP_003085667.1	hypothetical protein	-
PA1088_RS22790	4916204..4916629	-	426	WP_003163196.1	VOC family protein	-
PA1088_RS22795	4916730..4917614	+	885	WP_019725847.1	LysR family transcriptional regulator	-
PA1088_RS22800	4917587..4918540	-	954	WP_003085661.1	LysR family transcriptional regulator	-
PA1088_RS22805	4918761..4919195	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T61981 WP_015503432.1 NZ_CP015001:c4915200-4914835 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T61981 NZ_CP015001:c4915200-4914835 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT61981 WP_016561576.1 NZ_CP015001:c4914842-4914519 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT61981 NZ_CP015001:c4914842-4914519 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA