61917

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4732454..4733135 Replicon chromosome
Accession NZ_CP014948
Organism Pseudomonas aeruginosa strain N17-1

Toxin (Protein)


Gene name higB Uniprot ID V6AKP0
Locus tag OB07_RS21975 Protein ID WP_003111825.1
Coordinates 4732770..4733135 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1C7BMJ2
Locus tag OB07_RS21970 Protein ID WP_003159602.1
Coordinates 4732454..4732777 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OB07_RS21945 4728367..4729005 + 639 WP_003109444.1 hypothetical protein -
OB07_RS21950 4729248..4729583 + 336 WP_003119438.1 TM2 domain-containing protein -
OB07_RS21955 4729757..4730275 + 519 WP_012614469.1 PAAR domain-containing protein -
OB07_RS21960 4730272..4730973 + 702 WP_003111822.1 VRR-NUC domain-containing protein -
OB07_RS21965 4730990..4732078 + 1089 WP_062877683.1 DUF3396 domain-containing protein -
OB07_RS21970 4732454..4732777 - 324 WP_003159602.1 XRE family transcriptional regulator Antitoxin
OB07_RS21975 4732770..4733135 - 366 WP_003111825.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OB07_RS30280 4733426..4733665 - 240 WP_079388313.1 hypothetical protein -
OB07_RS21980 4733872..4734144 + 273 WP_015647856.1 hypothetical protein -
OB07_RS21985 4734175..4734600 - 426 WP_003111827.1 ring-cleaving dioxygenase -
OB07_RS21990 4734701..4735585 + 885 WP_003111828.1 LysR family transcriptional regulator -
OB07_RS21995 4735558..4736511 - 954 WP_003085661.1 LysR family transcriptional regulator -
OB07_RS22000 4736732..4737166 + 435 WP_003116494.1 RidA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-63)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13880.24 Da        Isoelectric Point: 4.8219

>T61917 WP_003111825.1 NZ_CP014948:c4733135-4732770 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Download         Length: 366 bp

>T61917 NZ_CP014948:c4733135-4732770 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11882.71 Da        Isoelectric Point: 6.4751

>AT61917 WP_003159602.1 NZ_CP014948:c4732777-4732454 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEGQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Download         Length: 324 bp

>AT61917 NZ_CP014948:c4732777-4732454 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGGACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6AKP0

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7BMJ2

References