Detailed information of TA system TA061910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5872383..5872963
• Replicon: chromosome
• Accession: NZ_CP014947
• Organism: Pseudomonas koreensis strain D26

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A554AH63
• Locus tag: AYO71_RS26380
• Protein ID: WP_041477766.1
• Coordinates: 5872383..5872661 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AYO71_RS26385
• Protein ID: WP_016773085.1
• Coordinates: 5872676..5872963 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYO71_RS29110	5867869..5868036	-	168	WP_016773090.1	DUF2474 domain-containing protein	-
AYO71_RS26365	5868121..5869128	-	1008	WP_041477769.1	cytochrome d ubiquinol oxidase subunit II	-
AYO71_RS26370	5869132..5870577	-	1446	WP_041477768.1	cytochrome ubiquinol oxidase subunit I	-
AYO71_RS26375	5871048..5872283	+	1236	WP_041477767.1	MFS transporter	-
AYO71_RS26380	5872383..5872661	+	279	WP_041477766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYO71_RS26385	5872676..5872963	+	288	WP_016773085.1	HigA family addiction module antidote protein	Antitoxin
AYO71_RS26390	5872968..5873519	-	552	WP_041477765.1	DJ-1/PfpI family protein	-
AYO71_RS26395	5873587..5874033	-	447	WP_041477764.1	DUF4879 domain-containing protein	-
AYO71_RS26400	5874254..5875549	+	1296	WP_016773083.1	NCS2 family permease	-
AYO71_RS26405	5875546..5876625	+	1080	WP_041477763.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
AYO71_RS26410	5876680..5877126	-	447	WP_041477762.1	DUF2442 domain-containing protein	-
AYO71_RS26415	5877123..5877551	-	429	WP_041477761.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-75)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10771.34 Da
• Isoelectric Point: 9.5056

>T61910 WP_041477766.1 NZ_CP014947:5872383-5872661 [Pseudomonas koreensis]
MIKSFQHKGLRGFYETGTTRGIRADHAKRLSRMLQFMDRATLPADLDLPGWRLHPLKGELSEYWSLSVSGNWRVIFRFVG
SDIELVDYLDYH

Nucleotide

• Length: 279 bp

>T61910 NZ_CP014947:5872383-5872661 [Pseudomonas koreensis]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTATGAAACAGGTACGACTCGTGGAATTCGTGCCGACCATGC
AAAACGGCTTTCGCGCATGTTGCAGTTCATGGATCGAGCGACGCTTCCCGCAGACCTGGACTTGCCCGGCTGGCGCCTGC
ATCCGTTGAAAGGTGAGCTGAGCGAATATTGGTCGCTCAGCGTTTCAGGCAACTGGCGAGTGATCTTTCGTTTTGTCGGT
TCGGATATCGAATTGGTCGATTATCTGGACTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10525.13 Da
• Isoelectric Point: 6.6962

>AT61910 WP_016773085.1 NZ_CP014947:5872676-5872963 [Pseudomonas koreensis]
MPMHNPPHPGETLLLDVLPELGISVSELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARVWLGVQTDYDLWQA
EHREQPMIEPLAAHG

Nucleotide

• Length: 288 bp

>AT61910 NZ_CP014947:5872676-5872963 [Pseudomonas koreensis]
ATGCCCATGCACAACCCGCCACACCCCGGTGAAACCCTGCTGCTGGATGTCTTGCCCGAGCTTGGTATCAGCGTGAGCGA
ATTGGCCCGGCACCTTGGCTTTGCACGCCCGCATCTTTCGCGCGTACTGCACGGACACGCGCCAATCAGCCCGGATCTGG
CAGTACGACTTGAGCGTGCAGGGATCGGCAAGGCGCGCGTTTGGCTAGGCGTCCAAACCGACTACGATCTCTGGCAAGCG
GAGCACCGTGAGCAACCAATGATAGAACCCCTCGCAGCCCACGGCTGA

Structures

Toxin

• AlphaFold DB: A0A554AH63