Detailed information of TA system TA061909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5604288..5604943
• Replicon: chromosome
• Accession: NZ_CP014947
• Organism: Pseudomonas koreensis strain D26

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A554AC95
• Locus tag: AYO71_RS25115
• Protein ID: WP_041479945.1
• Coordinates: 5604599..5604943 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AYO71_RS25110
• Protein ID: WP_041479944.1
• Coordinates: 5604288..5604602 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYO71_RS25085	5600065..5601237	-	1173	WP_041479939.1	aspartate aminotransferase family protein	-
AYO71_RS25090	5601340..5602260	+	921	WP_041479940.1	LysR family transcriptional regulator	-
AYO71_RS25095	5602299..5602703	+	405	WP_041479941.1	GNAT family N-acetyltransferase	-
AYO71_RS25100	5602837..5603538	+	702	WP_041479942.1	hypothetical protein	-
AYO71_RS25105	5603611..5604102	-	492	WP_041479943.1	RidA family protein	-
AYO71_RS25110	5604288..5604602	-	315	WP_041479944.1	helix-turn-helix domain-containing protein	Antitoxin
AYO71_RS25115	5604599..5604943	-	345	WP_041479945.1	hypothetical protein	Toxin
AYO71_RS25120	5605059..5605946	-	888	WP_016773305.1	heme o synthase	-
AYO71_RS25125	5605957..5606292	-	336	WP_041479946.1	cytochrome o ubiquinol oxidase subunit IV	-
AYO71_RS25130	5606292..5606915	-	624	WP_041479947.1	cytochrome o ubiquinol oxidase subunit III	-
AYO71_RS25135	5606919..5608949	-	2031	WP_041479948.1	cytochrome o ubiquinol oxidase subunit I	-
AYO71_RS25140	5608953..5609894	-	942	WP_024014231.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13523.49 Da
• Isoelectric Point: 10.3452

>T61909 WP_041479945.1 NZ_CP014947:c5604943-5604599 [Pseudomonas koreensis]
MRTIFFETTIFTKSVGRYLTDDEYRTLQNYLQSNPLAGDVMPRTGGFRKLRWADSRRGKGRRGGLRVLYYWLMNDGQFWM
FAIYDKDELEKLTAEQERALRTAIDKELKKRGTP

Nucleotide

• Length: 345 bp

>T61909 NZ_CP014947:c5604943-5604599 [Pseudomonas koreensis]
ATGCGAACAATCTTTTTTGAAACAACGATATTTACCAAAAGCGTTGGCCGCTATCTGACGGACGACGAATATCGAACATT
GCAGAACTACCTTCAGAGCAATCCGCTTGCGGGCGATGTAATGCCTCGAACGGGTGGCTTCCGTAAGCTGCGCTGGGCTG
ATTCTCGCCGTGGCAAGGGAAGACGAGGCGGCTTGCGGGTTCTCTACTATTGGCTTATGAATGATGGTCAGTTCTGGATG
TTCGCCATTTACGACAAAGATGAGCTTGAGAAGCTGACTGCCGAGCAAGAGAGGGCACTCAGGACAGCCATTGATAAAGA
GTTGAAAAAACGAGGTACCCCATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11900.82 Da
• Isoelectric Point: 9.9443

>AT61909 WP_041479944.1 NZ_CP014947:c5604602-5604288 [Pseudomonas koreensis]
MKKRDLFAELMQGVDEMAAQREGKITLRNTVLEDKPVPEVGAQEIVALREKLHMSQAVFASRIRTSPNTLRNWEQEKSKP
NAQAALLIRLVEKFPDMVERLNTV

Nucleotide

• Length: 315 bp

>AT61909 NZ_CP014947:c5604602-5604288 [Pseudomonas koreensis]
ATGAAAAAACGCGACCTGTTTGCAGAGTTAATGCAGGGCGTTGATGAGATGGCGGCTCAACGCGAGGGCAAGATCACACT
GCGCAATACAGTGCTTGAGGACAAGCCTGTACCTGAAGTGGGCGCACAGGAAATTGTGGCTCTTCGAGAAAAACTGCATA
TGTCTCAAGCTGTTTTTGCCAGTCGAATCAGGACAAGTCCAAACACCCTTCGAAATTGGGAGCAGGAAAAGTCGAAGCCC
AATGCTCAAGCAGCCTTATTGATACGACTGGTTGAAAAATTTCCAGATATGGTTGAGCGCTTGAACACTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A554AC95