Detailed information of TA system TA061902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 925769..926358
• Replicon: chromosome
• Accession: NZ_CP014947
• Organism: Pseudomonas koreensis strain D26

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A554ACX3
• Locus tag: AYO71_RS04125
• Protein ID: WP_041477483.1
• Coordinates: 926059..926358 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AYO71_RS04120
• Protein ID: WP_016772178.1
• Coordinates: 925769..926056 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYO71_RS04100	921623..922030	+	408	WP_016772182.1	RNA-binding protein S4	-
AYO71_RS04105	922128..922928	-	801	WP_041477480.1	phosphatase PAP2 family protein	-
AYO71_RS04110	923036..923938	+	903	WP_041477481.1	Hsp33 family molecular chaperone HslO	-
AYO71_RS04115	924120..925661	+	1542	WP_041477482.1	phosphoenolpyruvate carboxykinase	-
AYO71_RS04120	925769..926056	-	288	WP_016772178.1	helix-turn-helix domain-containing protein	Antitoxin
AYO71_RS04125	926059..926358	-	300	WP_041477483.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYO71_RS04130	926469..927611	-	1143	WP_041477484.1	hypothetical protein	-
AYO71_RS04135	927885..929813	+	1929	WP_041477485.1	RecQ family ATP-dependent DNA helicase	-
AYO71_RS04140	930027..931253	+	1227	WP_041477486.1	3-hydroxyacyl-CoA dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (38-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11104.75 Da
• Isoelectric Point: 10.2649

>T61902 WP_041477483.1 NZ_CP014947:c926358-926059 [Pseudomonas koreensis]
MSLLSQRVRELLDDDAYATFQKQLVANPSIGDVMEGTGGVRKTRVASKGHGKRGGARVIYYHFVSASQIALLMIYPKNEQ
QDLTHDERKALKAMIGNWR

Nucleotide

• Length: 300 bp

>T61902 NZ_CP014947:c926358-926059 [Pseudomonas koreensis]
ATGTCGCTGCTTTCGCAGCGAGTAAGAGAGTTACTGGACGACGATGCCTACGCCACCTTTCAAAAACAGTTGGTCGCAAA
TCCGTCTATTGGCGATGTAATGGAAGGAACCGGCGGGGTGCGTAAAACACGAGTCGCCTCCAAAGGCCATGGCAAACGGG
GTGGCGCACGGGTTATCTACTACCACTTCGTTTCAGCATCACAGATCGCGTTGTTGATGATTTACCCAAAAAACGAGCAG
CAGGATCTTACCCATGACGAACGGAAAGCACTGAAGGCCATGATTGGAAACTGGAGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10753.42 Da
• Isoelectric Point: 8.6214

>AT61902 WP_016772178.1 NZ_CP014947:c926056-925769 [Pseudomonas koreensis]
MSKFFDELMESVQEMDEILRGEKKPSREFYVDALQVKQIRKATGMTQAKFAALIDVQLGTLRNWEQGRRQPTGPAKALLK
AIHNDPVHVLSALAD

Nucleotide

• Length: 288 bp

>AT61902 NZ_CP014947:c926056-925769 [Pseudomonas koreensis]
ATGAGCAAGTTTTTTGATGAGCTGATGGAAAGCGTGCAGGAAATGGATGAGATTCTTCGGGGTGAAAAAAAGCCGTCCCG
CGAATTCTATGTGGATGCATTACAGGTTAAACAGATTCGCAAAGCGACGGGAATGACGCAGGCAAAGTTCGCAGCCTTGA
TTGATGTCCAGTTGGGAACGTTACGTAATTGGGAGCAAGGTCGTCGTCAGCCGACGGGGCCGGCCAAAGCACTACTCAAG
GCCATTCATAACGATCCTGTCCATGTGCTGAGTGCCCTGGCCGATTGA

Structures

Toxin

• AlphaFold DB: A0A554ACX3