Detailed information of TA system TA061858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5414282..5414877
• Replicon: chromosome
• Accession: NZ_CP014866
• Organism: Pseudomonas aeruginosa strain PA_154197

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N0KDU0
• Locus tag: AXW93_RS25750
• Protein ID: WP_023436306.1
• Coordinates: 5414599..5414877 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXW93_RS25745
• Protein ID: WP_003133769.1
• Coordinates: 5414282..5414587 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXW93_RS25690	5409355..5409606	-	252	WP_003115979.1	hypothetical protein	-
AXW93_RS25700	5409728..5410162	-	435	WP_025324669.1	hypothetical protein	-
AXW93_RS25710	5410678..5410968	-	291	WP_023088865.1	DUF5447 family protein	-
AXW93_RS25715	5410972..5411166	-	195	WP_080911835.1	hypothetical protein	-
AXW93_RS25720	5411180..5411452	-	273	WP_003115921.1	hypothetical protein	-
AXW93_RS25730	5411899..5412237	+	339	WP_058343476.1	hypothetical protein	-
AXW93_RS25735	5412242..5413312	-	1071	WP_080911836.1	reverse transcriptase family protein	-
AXW93_RS25740	5413388..5413606	-	219	WP_080911837.1	hypothetical protein	-
AXW93_RS25745	5414282..5414587	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
AXW93_RS25750	5414599..5414877	-	279	WP_023436306.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXW93_RS25760	5415206..5417434	+	2229	WP_043104653.1	TonB-dependent receptor	-
AXW93_RS25765	5417504..5418151	-	648	WP_003095021.1	carbonate dehydratase	-
AXW93_RS25770	5418213..5419451	-	1239	WP_023107901.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10588.11 Da
• Isoelectric Point: 7.2451

>T61858 WP_023436306.1 NZ_CP014866:c5414877-5414599 [Pseudomonas aeruginosa]
MILTFRCDEARQLFETGLSRQWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T61858 NZ_CP014866:c5414877-5414599 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGGCTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCAGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAACGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT61858 WP_003133769.1 NZ_CP014866:c5414587-5414282 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT61858 NZ_CP014866:c5414587-5414282 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A6N0KDU0