Detailed information of TA system TA061837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1609329..1609938
• Replicon: chromosome
• Accession: NZ_CP014841
• Organism: Dyella thiooxydans strain ATSB10

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: ATSB10_RS18925
• Protein ID: WP_083966132.1
• Coordinates: 1609329..1609607 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ATSB10_RS18930
• Protein ID: WP_083966133.1
• Coordinates: 1609627..1609938 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATSB10_RS07320	1605121..1607541	-	2421	WP_063671699.1	TonB-dependent receptor	-
ATSB10_RS18915	1607704..1608066	+	363	WP_083966130.1	helix-turn-helix transcriptional regulator	-
ATSB10_RS18920	1608213..1608467	+	255	WP_083966131.1	helix-turn-helix domain-containing protein	-
ATSB10_RS18925	1609329..1609607	+	279	WP_083966132.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ATSB10_RS18930	1609627..1609938	+	312	WP_083966133.1	HigA family addiction module antidote protein	Antitoxin
ATSB10_RS19325	1610658..1611575	-	918	WP_157469154.1	hypothetical protein	-
ATSB10_RS07325	1611758..1613440	-	1683	WP_063671701.1	DUF262 domain-containing protein	-
ATSB10_RS07330	1613442..1614485	-	1044	WP_063671703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1583574..1620999	37425

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (30-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10702.13 Da
• Isoelectric Point: 6.7014

>T61837 WP_083966132.1 NZ_CP014841:1609329-1609607 [Dyella thiooxydans]
MIHSVSGPLAKELYSGQCPKELELCRTTAERKLTYLDSVVTLEALRYPPGNRLEKLMGNRTGQYSIRINDQYRICFRWEN
DGPHDVEITDYH

Nucleotide

• Length: 279 bp

>T61837 NZ_CP014841:1609329-1609607 [Dyella thiooxydans]
ATGATCCACTCTGTATCAGGCCCCCTGGCTAAGGAGCTCTATTCCGGGCAGTGTCCTAAGGAACTCGAGCTATGCAGGAC
CACAGCCGAGCGCAAGCTTACCTACCTTGACTCCGTTGTTACGCTCGAAGCGCTTAGGTATCCACCTGGAAATCGACTAG
AAAAATTAATGGGAAATCGGACTGGTCAGTACAGCATCAGGATCAATGATCAGTATCGCATCTGCTTCCGTTGGGAGAAT
GACGGGCCGCATGATGTGGAGATAACCGACTACCATTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11612.43 Da
• Isoelectric Point: 7.4046

>AT61837 WP_083966133.1 NZ_CP014841:1609627-1609938 [Dyella thiooxydans]
MVKNGMRAIHPGEILREDFLPGYDMSPNALAQALRVPATRIHAIVNEKRAITADTALRLGRLFATGPDVWMNLQAKFDLR
KAEIEFGEIVEHDVRPIEQRHVG

Nucleotide

• Length: 312 bp

>AT61837 NZ_CP014841:1609627-1609938 [Dyella thiooxydans]
ATGGTTAAAAATGGCATGCGTGCCATTCATCCAGGGGAGATTCTCAGGGAAGATTTTCTCCCCGGCTATGACATGTCGCC
AAACGCCTTGGCACAGGCTCTTCGTGTTCCGGCGACTCGAATTCACGCGATTGTTAACGAGAAGCGAGCAATCACGGCGG
ATACAGCCCTACGTCTCGGCCGACTGTTCGCAACCGGCCCCGACGTGTGGATGAACTTGCAGGCAAAATTCGATCTACGT
AAAGCGGAAATTGAGTTTGGCGAAATTGTTGAGCATGATGTCCGCCCGATCGAGCAACGACACGTCGGTTAG