Detailed information of TA system TA061830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2991918..2992493
• Replicon: chromosome
• Accession: NZ_CP014839
• Organism: Pandoraea thiooxydans strain ATSB16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0G3ENY8
• Locus tag: PATSB16_RS13715
• Protein ID: WP_047214677.1
• Coordinates: 2992215..2992493 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: PATSB16_RS13710
• Protein ID: WP_047214676.1
• Coordinates: 2991918..2992205 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PATSB16_RS13690	2987401..2988429	-	1029	WP_083566776.1	porin	-
PATSB16_RS13695	2988610..2989947	-	1338	WP_047214674.1	MFS transporter	-
PATSB16_RS13700	2990077..2991069	+	993	WP_083566777.1	LysR family transcriptional regulator	-
PATSB16_RS13705	2991379..2991711	+	333	WP_047214675.1	type II toxin-antitoxin system HicB family antitoxin	-
PATSB16_RS13710	2991918..2992205	-	288	WP_047214676.1	HigA family addiction module antidote protein	Antitoxin
PATSB16_RS13715	2992215..2992493	-	279	WP_047214677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PATSB16_RS13720	2992945..2993997	+	1053	WP_083566884.1	alcohol dehydrogenase family protein	-
PATSB16_RS13725	2994141..2994713	+	573	WP_047214679.1	NAD(P)H-dependent oxidoreductase	-
PATSB16_RS21065	2995038..2995556	+	519	WP_206093650.1	hypothetical protein	-
PATSB16_RS13730	2995584..2996819	+	1236	WP_206093651.1	ATP-binding cassette domain-containing protein	-
PATSB16_RS13735	2996792..2997364	-	573	WP_047214680.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10406.76 Da
• Isoelectric Point: 10.0370

>T61830 WP_047214677.1 NZ_CP014839:c2992493-2992215 [Pandoraea thiooxydans]
MIKTFRHKGLEAFYRTGNKGGIQPRHAGKLRLLLTALDSAAGPQDMNAPAWRLHSLTGDLAGHWSIWVNGNWRVTFRFNG
ADAELVDYQDYH

Nucleotide

• Length: 279 bp

>T61830 NZ_CP014839:c2992493-2992215 [Pandoraea thiooxydans]
ATGATAAAGACCTTTCGCCATAAGGGGTTAGAAGCTTTTTATCGCACAGGTAACAAAGGGGGCATCCAGCCACGCCATGC
CGGAAAGTTGCGCTTGTTGCTGACCGCTCTGGACAGTGCCGCTGGTCCACAGGACATGAATGCGCCGGCGTGGCGCCTGC
ACTCGTTGACTGGTGATTTGGCTGGTCACTGGTCGATCTGGGTGAACGGCAATTGGCGCGTGACGTTCCGCTTCAACGGC
GCAGATGCCGAACTGGTCGATTATCAGGATTACCACTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10712.58 Da
• Isoelectric Point: 11.1366

>AT61830 WP_047214676.1 NZ_CP014839:c2992205-2991918 [Pandoraea thiooxydans]
MSKMHNPAHPGEVLRQWLPQDMTVTEAAQALQVSRITLSKVLNGKAGVTAGMALRLSAWLGTSPELWLGMQNQWDLWQAE
QQPKPKIKPLKRMRV

Nucleotide

• Length: 288 bp

>AT61830 NZ_CP014839:c2992205-2991918 [Pandoraea thiooxydans]
ATGAGCAAGATGCACAACCCCGCTCACCCCGGCGAAGTGTTGCGGCAATGGTTGCCGCAGGACATGACGGTCACGGAAGC
GGCCCAGGCGTTACAGGTCTCCCGCATTACGCTGTCCAAGGTGTTGAACGGCAAGGCAGGCGTGACTGCGGGCATGGCGT
TGCGCCTTTCGGCATGGCTGGGCACGTCGCCCGAGTTGTGGCTGGGGATGCAAAATCAGTGGGATCTGTGGCAGGCGGAG
CAGCAGCCAAAGCCGAAGATAAAGCCGCTCAAACGCATGCGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0G3ENY8