Detailed information of TA system TA061796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 85913..86570
• Replicon: plasmid pASP-a58
• Accession: NZ_CP014775
• Organism: Aeromonas veronii strain AVNIH1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: WM43_RS22130
• Protein ID: WP_000270043.1
• Coordinates: 86220..86570 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WM43_RS22125
• Protein ID: WP_000124640.1
• Coordinates: 85913..86215 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM43_RS22075	81538..81966	+	429	WP_000591074.1	hypothetical protein	-
WM43_RS22080	82023..82382	+	360	WP_000422768.1	hypothetical protein	-
WM43_RS22085	82382..82828	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
WM43_RS22090	82825..83343	+	519	WP_000210756.1	nitrite reductase	-
WM43_RS22095	83343..83573	+	231	WP_000972663.1	hypothetical protein	-
WM43_RS22100	83560..84417	+	858	WP_001167032.1	hypothetical protein	-
WM43_RS22110	84648..85175	+	528	WP_001236377.1	thermonuclease family protein	-
WM43_RS22115	85233..85505	+	273	WP_001043047.1	HU family DNA-binding protein	-
WM43_RS22120	85593..85886	+	294	WP_001239997.1	chromosome segregation protein ParM	-
WM43_RS22125	85913..86215	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
WM43_RS22130	86220..86570	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WM43_RS22135	86733..87281	+	549	WP_001061574.1	transcriptional regulator	-
WM43_RS22145	87622..87816	+	195	WP_000343597.1	hypothetical protein	-
WM43_RS22150	87827..88198	+	372	WP_000516916.1	hypothetical protein	-
WM43_RS22155	88191..88661	+	471	WP_001281821.1	hypothetical protein	-
WM43_RS22160	88676..89011	-	336	WP_000683476.1	hypothetical protein	-
WM43_RS22165	89108..89596	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
WM43_RS22170	89599..90096	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / aadA2 / dfrA12 / qnrB4 / ere(A) / aac(6')-IIc / blaSHV-12 / tet(D) / floR / sul2	-	1..198307	198307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T61796 WP_000270043.1 NZ_CP014775:c86570-86220 [Aeromonas veronii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T61796 NZ_CP014775:c86570-86220 [Aeromonas veronii]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT61796 WP_000124640.1 NZ_CP014775:c86215-85913 [Aeromonas veronii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT61796 NZ_CP014775:c86215-85913 [Aeromonas veronii]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9